Parasitology Research

, Volume 77, Issue 1, pp 59–64 | Cite as

Monoclonal antibodies identify micronemes and a new population of cytoplasmic granules cross-reacting with micronemes of cystozoites ofSarcocystis muris

  • R. Entzeroth
  • A. König
  • J. -F. Dubremetz
Original Investigations


Micronemes of cystozoites ofSarcocystis muris were isolated after subcellular fractionation and used for immunization of BALB/C mice. After spleen cells of immunized mice were fused with SP20 myeloma cells, ten different monoclonal antibodies (mAbs) were isolated. These antibodies reacted with antigens whose molecular weight ranged from 16 to >90 kDa. Six mAbs recognized granules of 150–400 nm that were located in the vicinity of the Golgi complex but were not identical with dense granules. Two mAbs (2A3, 3A8) were specific for micronemes of cystozoites as demonstrated by immunoelectron microscopy. However, these antibodies also recognized the population of granules near the Golgi complex. Cross-reactivity between micronemes and a dense granule population has not previously been reported. Host cells that had been contacted by cystozoites showed patchy fluorescence when probed with mAb 2A3. This suggests that microneme antigens could be transferred to the host-cell surface during parasitehost cell interactions.


Molecular Weight Monoclonal Antibody Host Cell Myeloma Spleen Cell 
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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • R. Entzeroth
    • 1
  • A. König
    • 1
  • J. -F. Dubremetz
    • 2
  1. 1.Zoologisches InstitutBonn 1Federal Republic of Germany
  2. 2.U 42INSERMVilleneuve-D'AscqFrance

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