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Detection of early pregnancy factor (EPF) in pregnant and nonpregnant subjects with the rosette inhibition test

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Summary

We tested for early pregnancy factor (EPF) using a rosette inhibition test with polyclonal anti-lymphocyte serum from the horse and two monoclonal antibodies specific for the E-receptor of T-lymphocytes. When lymphocytes were preincubated with early human pregnancy sera, rosette inhibition titres were four or more dilutions higher (P<0.01) than when lymphocytes were preincubated with nonpregnant sera.

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Authors and Affiliations

  1. Forschungszentrum für Tierproduktion Dummerstorf-Rostock der Akademie der Landwirtschaftswissenschaft der DDR, GDR

    U. Tiemann

  2. Klinik für Gynäkologie und Geburtshilfe der Ernst-Moritz-Arndt-Universität, Wollweberstrasse 1, DDR-2200, Greifswald

    W. Straube, M. Loh & M. Schütz

Authors
  1. W. Straube
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  2. U. Tiemann
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  3. M. Loh
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  4. M. Schütz
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Straube, W., Tiemann, U., Loh, M. et al. Detection of early pregnancy factor (EPF) in pregnant and nonpregnant subjects with the rosette inhibition test. Arch Gynecol Obstet 246, 181–187 (1989). https://doi.org/10.1007/BF00934079

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  • DOI: https://doi.org/10.1007/BF00934079

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