Schistosoma mansoni infection of Syrian golden hamsters: The host humoral immune response in relation to the adult worm burdens after primary infection
- 28 Downloads
Seven-week-old female Syrian golden hamsters (Mesocricetus auratus) showed different degrees of susceptibility toSchistosoma mansoni, as assessed by the percentage of cercariae recovered as adult worms 6 weeks after infection. Plasma of the low (A), medium (B) and high (C) susceptibility groups were tested immunochemically. No differences were observed in the concentrations of albumin, α1-, α2-, ;β- and γ-globulins as measured by cellulose acetate electrophoresis. However, a significantly higher percentage of animals in groups A and B than in group C had anS. mansoni specific “beforked” IgG precipitin band and specific antibodies against a worm tegumental antigen preparation (AWT).
Conversely, more animals in group C made antibodies against a “denuded” worm-body antigen preparation (AWB) than in groups A and B. However, by using the enzyme-linked immunosorbent assay, no significant differences in antibody titres against AWT, AWB and a total worm antigen (AVA) were observed in the animals in groups A, B and C.
Upon consideration of the immunochemical data in relation to the distribution pattern of susceptibility to infection, we propose that the intensity ofS. mansoni infection in the hamster is a polygene-controlled phenomenon and depends upon the presentation of differing parasite antigenic component(s) to the host.
KeywordsCellulose Acetate Humoral Immune Response Adult Worm Schistosoma Worm Burden
Unable to display preview. Download preview PDF.
- Araj GF, Matossian RM, Frayha GJ (1977) The host response in secondary hydatidosis of mice. I. Circulating antibodies. Z Parasitenkd 52:23–30Google Scholar
- Bienenstock J, Block KJ (1970) Immunoglobulins of the hamster. I. Antibody activity in four immunoglobulin classes. J Immunol 104:1220–1229Google Scholar
- Das PK, Aalberse RC, Eck S van, Iskander R, Boelens P, Hilditch TJO (1980) Subcellular fraction of adultSchistosoma mansoni: potential for diagnosis; Abstract, Spring Meeting of Netherlands Society of Parasitology, Trop Geogr Med 32:p. 364Google Scholar
- Ceska M (1968) Antigen-antibody interaction in the presence of water suluble non-ionic resins. Biochem Biophys Acta 177:625–628Google Scholar
- Cheever AW (1965) A comparative study ofSchistosoma mansoni infections in mice, gerbils, multimammate rats and hamsters. II. Qualitative pathological differences. Am J Trop Med Hyg 14:227–238Google Scholar
- Hillyer GV, Lewert RM (1974) Studies on renal pathology in hamsters infected withSchistosoma mansoni andS. japonicum. Am J Trop Med Hyg 23:404–411Google Scholar
- Iskander R, Das PK, Aalberse RC (1981) IgG4 antibodies in Egyptian patients with Schistosomiasis. Int Arch Allergy appl Immunol 66:200–207Google Scholar
- Kuntz RE, Stirewalt MA (1946) Effect of DDT on cercariae ofSchistosoma mansoni. J Parasitol 32:529–538Google Scholar
- Kusel JR (1972) Protein composition and protein synthesis in the surface membranes ofSchistosoma mansoni. Parasitology 65:55–69Google Scholar
- Lichtenberg F van, Erickson DG, Sadun EH (1973) Comparative histopathology of schistosome granulomas in hamsters. Am J Path 72:149–177Google Scholar
- Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. Biol Chem 193:265–275Google Scholar
- Mitchell GF, Rajasekariah GR, Rickard MD (1980) A mechanism to account for mouse strain variation in resistance to the larval cestode,Taenia taeniaeformis. Immunology 39:481–489Google Scholar
- Morrison WI, Roelants GE, Mayor-Whitney KS, Murray M (1978) Susceptibility of inbred mice toTrypanosoma congolense: correlation with changes in spleen lymphocyte populations. Clin Exp Immunol 32:25–40Google Scholar
- Purnell WP (1966) Host-parasite relationships in Schistosomiasis. II. The effects of age and sex on the infection of mice and hamsters with cercariae ofSchistosoma mansoni and of hamsters with cercariae ofSchistosoma haematobium. Ann Trop Med Parasit 60:94–99Google Scholar
- Ruitenberg EJ, Steerenberg PA, Brosi BJM, Buys J (1976) Reliability of the enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis ofTrichinella spiralis infections in conventionally raised pigs. J Immunol Meth 10:67–83Google Scholar
- Smith I (1968) Alkaline phosphatase. Chromatographic and electrophoretic techniques. In: Smith, I. (ed) Starch gel electrophoresis, 2nd edn. William Heinemann Medical Books Ltd., London, p 271Google Scholar
- Smith MA, Clegg JA (1976) Different levels of acquired immunity to schistosoma in two strains of hamsters; Parasitology 73:47–52Google Scholar
- Smith MA, Clegg JA, Kusel JR, Webbe G (1975) Lung inflammation in immunity toSchistosoma mansoni. Experientia 31:595–596Google Scholar
- Smithers SR, Terry RJ (1965) The infection of laboratory hosts with cercariae ofSchistosoma mansoni and the recovery of adult worms. Parasitology 55:695–700Google Scholar
- Smithers SR, Terry RJ (1969) The immunology of Schistosomiasis. In: Dawes B (ed) Advances in Parasitology, vol 7. Academic Press, New York, pp 41–93Google Scholar
- Smithers SR, Terry RJ (1976) The immunology of Schistosomiasis. In: Dawes B (ed) Advances in Parasitology vol. 14. Academic Press, New York, pp 399–422Google Scholar
- Stiffel C, Mouton D, Bouthiller Y, Heuman AM, Decreusefond C, Mevel JC Biozzi G (1974) Polygenic regulation of general antibody synthesis in the mouse. In: Brent L, Holborrow J (eds) Progress in Immunology II, vol 2. North-Holland, Amsterdam, pp 203–212Google Scholar
- Stirewalt MA, Kuntz RE, Evans AS (1951) The relative susceptibilities of the commonly-used laboratory mammals to infection bySchistosoma mansoni. Am J Trop Med Hug 31:57–82Google Scholar
- Warren KS, Peters PA (1967) Comparison of penetration and maturation ofSchistosoma mansoni in the hamster, mouse, guinea pig, rabbit, and rat. Am J Trop Med Hyg 16:718–722Google Scholar
- Yong WK, Heath DD (1979) “Are 5” antibodies in sera of sheep infected withEchinococcus granulosus, Taenia hydatigena andTaenia ovis. Par Immunol 1:27–38Google Scholar