Abstract
Use of the fluorescence-activated cell sorter proved to be an accurate and highly efficient means for cloningBabesia parasites. These qualities were examined by separating a mixed population ofBabesia-infected bovine erythrocytes composed of two isolates with different karyotypes. Direct evidence of polymorphism was detected during comparison of the resultant clones.
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Bianco AE, Battye FL, Brown GV (1986)Plasmodium falciparum: rapid quantification of parasitemia in fixed malaria cultures by flow cytometry. Exp Parasitol 62:275–282
Callow LL, Mellors LT, McGregor W (1979) Reduction in virulence ofBabesia bovis due to rapid passage in splenectomized cattle. Int J Parasitol 9:333–338
Carson CA, Timms P, Cowman AF, Stewart NP (1990)Babesia bovis: evidence for selection of subpopulations during attenuation. Exp Parasitol 70:404–410
Corocoran LM, Forsyth KP, Bianco AE, Brown GV, Kemp DJ (1986) Chromosome size polymorphisms inPlasmodium falciparum can involve deletions and are frequent in natural parasite populations. Cell 44:87–95
Cowman AF, Timms P, Kemp DJ (1984) DNA polymorphisms and subpopulations inBabesia bovis. Mol Biochem Parasitol 1:91–103
Dalrymple BP (1992) Diversity and selection inBabesia bovis and their impact on vaccine use. Parasitol Today 8:21–23
Erp EE, Ristic M, Carson CA (1978) Fluorscein diacetate staining ofAnaplasma marginale as a possible measure of viability. Am J Vet Res 39:45–46
Gill AC, Cowman AF, Stewart NP, Kemp DJ, Timms P (1987)Babesia bovis: molecular and biological characteristics of cloned parasite lines. Exp Parasitol 63:180–188
McElwain TF, Perryman LE, Davis WC, McGuire TC (1987) Antibodies define multiple proteins with epitopes exposed on the surface of liveBabesia bigemina merozoites. J Immunol 138:2298–2304
Ray BK, Bailey CW, Jensen JB, Carson CA (1992) Chromosomes ofBabesia bovis andBabesia bigemina. Mol Biochem Parasitol 52:123–126
Rodriguez SD, Buening GM, Green TJ, Carson CA (1983) Cloning ofBabesia bovis by in vitro cultivation. Infect Immun 42:15–18
Rosario V (1981) Cloning of naturally occurring mixed infection of malaria parasites. Science 212:1037–1038
Trager W, Tershakovec M, Lyardvert L, Stanley H, Lanners N, Gubert E (1981) Clones of the malaria parasite,Plasmodium falciparum obtained by microscopic selection: their characterization with regard to knobs, chloroquine sensitivity and formation of gametocytes. Proc Natl Acad Sci USA 78:6527–6530
Vega CA, Buening GM, Green TJ, Carson CA (1985) In vitro cultivation ofBabesia bigemina. Am J Vet Res 46:416–420
Vega CA, Buening GM, Rodriguez SD, Carson CA (1986) Cloning of in vitro propagatedBabesia bigemina. Vet Parasitol 22:223–233
Welsh J, McClelland M (1990) Fingerprinting genomes using PCE with arbitrary primers. Nucleic Acids Res 18:7213–7218
Wyatt CR, Goff W, Davis WC (1991) A flow cytometric method for assessing viability of intraerythrocytic hemoparasites. J Immunol Methods 140:23–30
Yu K, Pauls KP (1992) Optimization of the PCR program for RAPD analysis. Nucleic Acids Res 20:2606
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Estes, D.M., Bailey, C.W., Barnett, L. et al. Fluorescence-activated cell sorting-derived clones ofBabesia bigemina show karyotype polymorphism. Parasitol Res 80, 104–107 (1994). https://doi.org/10.1007/BF00933775
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DOI: https://doi.org/10.1007/BF00933775