Summary
The response of a salt-tolerantWestiellopsis prolifica ARM 366 and a sensitiveAnabaena C-10 to NaCl was studied. While the former could tolerate up to 400mm NaCl, the latter was highly sensitive to concentrations above 50mm NaCl. Under salt stress, the tolerantW. prolifica showed an increased nitrogen demand as exemplified by high nitrogenase activity, and growth inhibition at higher concentrations of NaCl did not appear to be a direct consequence of inhibition of nitrogen fixation. One of the striking responses to Na+ challenge by the tolerantW. prolifica was the excess production of extracellular polysaccharides which adsorbed the bulk of the Na+. The influx of Na+ into the cell was comparatively small.
Résumé
On a étudié la réponse au NaCl d'une souche halo-tolérante deWestiellopsis prolifica (ARM 366) et, d'une souche halo-sensible d'Anabaena (C-10). Alors que la première tolère jusqu'a 400mm NaCl, la seconde est hautement sensible aux concentrations supérieures à 50mm. Soumise à un choc de salinité, la souche halo-tolérante deW. prolifica présente une demande d'azote accrue, comme le montre une activité nitrogénase élevée, et l'inhibition de la croissance par une forte concentration en NaCl ne parait pas être la conséquence directe d'une inhibition de la fixation d'azote. Une réponse remarquable deW. prolifica à l'agression par Na+ est la production en excès de polysaccharides exo-cellulaires, qui absorbent la plus grande partie du Na+. La pénétration de Na+ dans la cellule est relativement faible.
Resumen
Se estudió la respuesta al NaCl deWestiellopsis prolifica ARM 366, organismo tolerante a la sal, y deAnabaena C-10 que es sensible. El primero pudo tolerar concentraciones de hasta 400mm NaCl, sin embargo Anabaena C-10 se mostró extremadament sensible a concentraciones superiores a 50mm de NaCl. Bajo condiciones de estrés salinoW. prolifica mostró un incremento en la demanda de N2, como se deduce de la elevada actividad nitrogenásica; la inhibición del crecimiento a concentraciones superiores de NaCl no se pudo relacionar directamente con la inhibición de la fijación de nitrógeno. Una de las respuestas más sorprendentes deW. prolifica frente al Na+ fue la producción de un exceso de polisaccaridos extracelulares que absorbieron gran parte del Na+. El flujo de Na+ hacia la célula fue, pues, comparativemente pequeño.
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Jha, M.N., Venkataraman, G.S. & Kaushik, B.D. Response ofWestiellopsis prolifica andAnabaena sp. to salt stress. Mircen Journal 3, 307–317 (1987). https://doi.org/10.1007/BF00933584
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DOI: https://doi.org/10.1007/BF00933584