Isolation and characterization of variants ofRhizobium leguminosarum bvphaseoli

  • J. Rodriguez
  • J. R. Jardim Freire
  • I. Schrank
Research Papers


Seven spontaneous variants ofR. leguminosarum byphaseoli were isolated from three stock cultures by their diversity in colony morphology. Five variants had small opaque or translucent colonies and two variants, large gummy colonies. There were marked differences between strains, but not between variants of the same strain, in the utilization of carbon sources. The large gummy colony variants were more tolerant to streptomycin and novobiocin and less effective or ineffective in N2 fixation than the variants of small non-gummy colonies; they were also of higher competitive ability than the other variants from the same strain. At least for one strain, competitiveness seemed less subject to variation.


Carbon Source Streptomycin Competitive Ability Culture Stock Colony Morphology 
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A partir de cultivos de referencia, y debido a la diversidad morfológico de sus colonias, se aislaron siete variantes espontaneas deR. leguminosarum bvphaseoli. Cinco de las variantes presentaron colonias pequeñas, opacas o traslucidas. Las colonias de las otras dos variantes eran grandes y de aspecto gomoso. En la utilización de fuentes de carbono se observaron diferencias marcadas entre distintas cepas pero no entre variantes de la misma cepa. Las colonias grandes y de aspecto gomoso eran más tolerantes a la estreptomicina y a la novobiocina, menos eficaces que las variantes de colonias pequeñas en cuanto a la fijación de N2, y mostraron además una mayor habilidad competitiva frente a las otras variantes de la misma cepa. Al menos en una de las cepas la competitividad estaba menos sujeta a variaciones.


Sept variants spontanés deR. leguminosarum bvphaseoli ont été isolés à partir de trois cultures stock sur la base de la diversité de la morphologie de leurs colonies. Cinq variants se présentaient en colonies petites, opaques à translucides, trandis que deux variants se présentaient en grandes colonies visqueuses. Il y avait des différences marquées entre souches mais pas entre variants de la même souche, quant à l'utilisation de sources carbonées. Les variants se présentant en grandes colonies visqueuses étaient plus tolérants à la streptomycine et à la novobiocine et moins efficaces voire inefficaces pour la fixation de l'N2, que les variants des petites colonies non visqueuses; elles présentaient aussi une capacité de compétition plus élevée que les autres variants de la même souche. Au moins pour une souche, la capacité de compétition semblait moins sujette à variation.


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Copyright information

© Oxford University Press 1987

Authors and Affiliations

  • J. Rodriguez
    • 1
  • J. R. Jardim Freire
    • 2
  • I. Schrank
    • 3
  1. 1.Escuela Nacional de Ciencia BiologicasI.P.N.Mexico
  2. 2.Departamento de SolosFaculdade de Agronomia da UFRGSPorto AlegreBrazil
  3. 3.Centro de BiotecnologiaUFRGSPorto AlegreBrazil

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