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Parasitology Research

, Volume 76, Issue 5, pp 373–378 | Cite as

Increase of DNA content in tissue stages ofEntamoeba histolytica strain SFL 3

  • Ute Mackenstedt
  • Marita Schmidt
  • Wolfgang Raether
  • Heinz Mehlhorn
  • Manfred Uphoff
Original Investigations

Abstract

The DNA content of culture forms and tissue stages of pathogenicE. histolytica strain SFL 3 were measured photometrically after the nuclei had been stained with the fluorochrome BAO. As a control, the DNA guartity ofE. histolytica strain HK 9 andE. invadens were determined by the same method and compared with reference values. Tissue stages were obtained from hamsters experimentally infected by intrahepatic injection of SFL 3 amoebae. Further studies concerning possible changes in the DNA content of tissue stages involved the following methods: (a) isolation of tissue stages from the liver, followed by distinct suspension periods. (b) Infected liver pieces were directly transferred into culture medium; amoebae emigrating therefrom were cultivated. The study demonstrated that tissue stages contained up to 4 times more DNA than did culture forms. After 3 h cultivation, the DNA content of tissue stages decreased to the level of culture forms. Possible reasons for this change are discussed.

Keywords

Culture Form Infected Liver Tissue Stage Liver Piece Histolytica Strain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Ute Mackenstedt
    • 1
  • Marita Schmidt
    • 1
  • Wolfgang Raether
    • 2
  • Heinz Mehlhorn
    • 1
  • Manfred Uphoff
    • 2
  1. 1.Lehrstuhl für Spezielle Zoologie und ParasitologieRuhr-Universität BochumBochumGermany
  2. 2.Labor für Protozoologie und MykologieHoechst AGFrankfurt/M. 80Germany

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