Parasitology Research

, Volume 76, Issue 5, pp 373–378 | Cite as

Increase of DNA content in tissue stages ofEntamoeba histolytica strain SFL 3

  • Ute Mackenstedt
  • Marita Schmidt
  • Wolfgang Raether
  • Heinz Mehlhorn
  • Manfred Uphoff
Original Investigations


The DNA content of culture forms and tissue stages of pathogenicE. histolytica strain SFL 3 were measured photometrically after the nuclei had been stained with the fluorochrome BAO. As a control, the DNA guartity ofE. histolytica strain HK 9 andE. invadens were determined by the same method and compared with reference values. Tissue stages were obtained from hamsters experimentally infected by intrahepatic injection of SFL 3 amoebae. Further studies concerning possible changes in the DNA content of tissue stages involved the following methods: (a) isolation of tissue stages from the liver, followed by distinct suspension periods. (b) Infected liver pieces were directly transferred into culture medium; amoebae emigrating therefrom were cultivated. The study demonstrated that tissue stages contained up to 4 times more DNA than did culture forms. After 3 h cultivation, the DNA content of tissue stages decreased to the level of culture forms. Possible reasons for this change are discussed.


Culture Form Infected Liver Tissue Stage Liver Piece Histolytica Strain 
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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Ute Mackenstedt
    • 1
  • Marita Schmidt
    • 1
  • Wolfgang Raether
    • 2
  • Heinz Mehlhorn
    • 1
  • Manfred Uphoff
    • 2
  1. 1.Lehrstuhl für Spezielle Zoologie und ParasitologieRuhr-Universität BochumBochumGermany
  2. 2.Labor für Protozoologie und MykologieHoechst AGFrankfurt/M. 80Germany

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