Abstract
Effects of various gabergic anthelmintics on the guanidine-induced twitch responses in isolated frog rectus preparations were examined. All gabergic anthelmintics such as milbemycin oxime, milbemycin D, avermectin B1a, invermectin, and diethylcarbamazine (DEC) showed stimulatory effects on the guanidine-induced twitch responses at their higher concentrations. Only piperazine caused inhibitory effects on the twitch responses, even at higher concentrations. The stimulation of the twitch responses by the gabergic anthelmintics was antagonized with tetrodotoxin, hemicholinium-3, dtubocurarine, and strychnine. These results suggest that all gabergic anthelmintics except piperazine stimulate the release of acetylcholine from the nerve endings and that all of them, including piperazine, have different effects on the gabergic mechanism at lower concentrations and on the cholinergic mechanism at higher concentrations.
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References
Akao N, Konishi K, Kondo K (1991) Anthelmintic effects of milbemycin oxime on intestinal parasites of dogs and cats (in Japanese). J Vet Med 44:653–657
Aubry ML, Cowell P, Davey MJ, Shevde S (1970) Aspects of the pharmacology of a new anthelmintic: pyrantel. Br J Pharmacol 38:332–344
Bueding E, Bennett J (1972) Neurotransmitters in trematodes. In: Van den Bossche H (ed) Comparative biochemistry of parasites. Academic Press, New York London, pp 95–99
Del Castillo J, Morales T (1969) Electrophysiological experiments inAscaris lumbricoides. In: Kerkut GA (ed) Experiments in physiology and biochemistry, vol 2. Academic Press, New York, pp 209–273
Kass IS, Larsen DA (1982)Ascaris suum: differential effects of avermectin B1a on the intact animal and neuromuscular strip preparations. Exp Parasitol 54:166–174
Lee HH, Terada M (1992) In vitro effects of milbemycin oxime: mechanism of action againstAngiostrongylus cantonensis andDirofilaria immitis. Parasitol Res 78:349–353
Lee HH, Sahara K, Terada M (1991) In vitro effects of milbemycin oxime: effects on the motility of various parasitic helminths. Jpn J Parasitol 40:424–431
Martin RJ, Pennington AJ, Duittoz AH, Robertson S, Kusel JR (1991) The physiology and pharmacology of neuromuscular transmission in the nematode parasite,Ascaris suum. Parasitology 102:S41-S58
Mellanby H (1955) The identification and estimation of acetylcholine in three parasitic nematodes (Ascaris lumbricoides, Litomosoides carinii, and the microfilaria ofDirofilaria immitis). Parasitology 45:287–294
Natoff IL (1969) The pharmacology of the cholinoceptor in muscle preparations ofAscais lumbricoides varsuum. Br J Pharmacol 37:251–257
Norton S, De Beer EJ (1957) Investigations on the action of piperazine onAscaris lumbricoides. Am J Trop Med Hyg 6:898–905
Otsuka M, Endo M (1960) The effect of guanidine on neuromuscular transmission. J Pharmacol Exp Ther 128:273–282
Pax PA, Bennett JL (1991) Neurobiology of parasitic plat helminths: possible solution to the problem of correlating structure with function. Parasitology 102:S31-S39
Taniyama K, Saito N, Kusunoki M, Tanaka C (1987) Regulation mechanism of enteric nervous system in gastrointestinal motility. Autonomic Nervous System 24:310–316
Terada M, Sano M (1985) Studies on chemotherapy of parasitic helminths. XX. Neuropharmacological action of santonin onAngiostrongylus cantonensis and other parasitic nematodes (in Japanese). Jpn J Parasitol 34:325–334
Terada M, Sano M (1986) Effects of diethylcarbamazine on the motility ofAngiostrongylus cantonensis andDirofilaria immitis. Z Parasitenkd 72:375–385
Terada M, Ishii AI, Kino H, Sano M (1982) Studies on chemotherapy of parasitic helminths. VII. Effects of various cholinergic agents on the motility ofAngiostrongylus cantonensis. Jpn J Pharmacol 32:633–642
Terada M, Ishii AI, Kino H, Sano M (1983) Studies on chemotherapy of parasitic helminths. XVIII. Mechanism of spastically paralyzing action of pyrantel inAngiostrongylus cantonensis. Experientia 39:1383–1385
Terada M, Ishii AI, Kino H, Sano M (1984)Angiostrongylus cantonensis: paralysis due to avermectin B1a and ivermectin. Exp Parasitol 57:149–157
Terada M, Dharejo AM, Sano M (1986) Effects of milbemycin D on the motility ofAngiostrongylus cantonensis andA. costaricensis. Jpn J Parasitol 35:497–504
Turner MJ, Schaeffer JM (1989) Mode of action of ivermectin. In: Campbell WC (ed) Ivermectin and abamectin, Springer, Berlin Heidelberg New York, pp 73–88
Webster LT Jr (1990) Chemotherapy of parasitic infection. In: Gilman AG, Rall TW, Nies AS, Taylor P (eds) Goodman and Gilman's The pharmacological basis of therapeutics, 8th edn. Pergamon, New York, pp 954–977
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Terada, M., Chen, W., Wang, H.H. et al. Effects of gabergic anthelmintics at higher concentrations on the guanidine-induced twitch responses in isolated frog recturs preparations. Parasitol Res 80, 575–580 (1994). https://doi.org/10.1007/BF00933005
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DOI: https://doi.org/10.1007/BF00933005