Parasitology Research

, Volume 75, Issue 2, pp 123–131 | Cite as

Histochemical study ofSarcocystis sp. intramuscular cysts in gastrocnemius and soleus of the cat

  • Mario G. Fiori
  • Herbert E. Lowndes
Original Investigations

Abstract

Histochemical investigations ofSarcocystis microcysts found in two hindleg muscles of cats were carried out. Genus identification was based on the reinforced cyst membrane structure and its dimensions, the structure of the sarcocysts, and an electron microscopic survey of bradyzoite characteristics. The cyst membrane is partly contributed by the host myofiber, the characteristic histochemical features of which it retains. Materials adjacent to the limiting membrane make it appear thicker than it actually is, particulary when the PAS method as well as techniques for the demonstration of α-glycerophosphate dehydrogenase and ATPase activities are used. The ground substance occupying the parasitophorous vacuole is not amorphous and metabolically inert, but rather displays a fairly strong and definite ATPase activity, suggesting a trophic role in the support of metrocytes and zoites embedded therein. Cysts tend to adapt their biochemical characteristics to the particular metabolism of the muscle fibers in which they are located. All of these findings are discussed in terms of host-parasite relationships.

Keywords

ATPase Activity Biochemical Characteristic Cyst Membrane Genus Identification Histochemical Study 

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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Mario G. Fiori
    • 1
  • Herbert E. Lowndes
    • 1
  1. 1.Department of PharmacologyUMDNJ-New Jersey Medical SchoolNewarkUSA

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