Abstract
We carried out a quantitative and qualitative analysis of intestinal digestion of neutral lipids in suckling rats infected withGiardia duodenalis. Total lipids were measured after extraction from the contents of the stomach, proximal and distal small bowel, caecum and colon. Amounts gradually fell from the stomach to the colon and were identical in infected animals and controls, although high values were occasionally found in the caecum of infected rats. Relative glyceride quantities were determined by means of high-performance thin-layer chromatography. Triglycerides were absent from the distal small bowel, and only free fatty acids and cholesterol were present in the caecum, reflecting normal digestion of neutral lipids in infected suckling rats. Our results suggest thatG. duodenalis does not impair intestinal fat digestion in suckling rats.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barbieri D, De Brito T, Hoshino S, Nascimento OB, Martins Campos JV, Quarentei G, Marcondes E (1970) Giardiasis in childhood. Absorption tests and biochemistry, histochemistry, light, and electron microscopy of jejunal mucosa. Arch Dis Child 45: 466–472
Chochillon C, Gobert JG, Buchel L, Savel J (1986) Giardia intestinalis: étude comparée des débits fécaux lipidiques et azotés chez des adultes et des enfants parasités. Pathol Biol (Paris) 34: 830–834
Farthing MJG, Varon SR, Keusch GI (1983) Mammalian bile promotes growth ofGiardia lamblia in axenic culture. Trans R Soc Trop Med Hyg 77: 467–469
Favennec L, Chochillon C, Meillet D, Magne D, Savel J, Raichvarg D, Gobert JG (1990) Adherence and multiplication ofGiardia duodenalis on human enterocytes in vitro. Parasitol Res 76: 581–584
Folch J, Lees M, Sloane Stanley GH (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226: 497–509
Halliday CEW, Webb JPW, Casewell M, Farthing MJG (1986) Mechanism of bile salts uptake byGiardia lamblia and its possible relevance to intestinal disease. Gastroenterology 90: 1448
Halliday CEW, Clark C, Farthing MJG (1988)Giardia-bile salt interactions in vitro and in vivo. Trans R Soc Trop Med Hyg 82: 428–432
Hartong WA, Gourley WK, Arvanitakis C (1979) Giardiasis: clinical spectrum and functional-structural abnormalities of the small intestinal mucosa. Gastroenterology 77: 61–69
Jin M, Snider MD (1993) Role of microtubules in transferrin receptor transport from the cell surface to endosomes and the Golgi complex. J Biol Chem 268: 18390–18397
Katelaris PH, Farthing MJG (1992) Diarrhoea and malabsorption in giardiasis: a multifactorial process? Gut 33: 295–297
Katelaris P, Seow F, Ngu M (1991) The effects ofGiardia lamblia trophozoites on lipolysis in vitro. Parasitology 103: 35–39
Magne D, Favennec L, Chochillon C, Gorenflot A, Meillet D, Kapel N, Raichvarg D, Savel J, Gobert JG (1991) Role of cytoskeleton and surface lectins inGiardia duodenalis attachment to CaCO2 cells. Parasitol Res 77: 659–662
Maxwell RJ, Yeisley SW, Unruh J (1990) The importance of effective predevelopment plate washing techniques in thin-layer chromatography. J Liq Chromatogr 13: 2001–2011
Reaven EP, Reaven GM (1977) Distribution and contents of microtubules in relation to the transport of lipids: an ultrastructural quantitative study of the absorptive cell of the small intestine. J Cell Biol 75: 559–572
Seow F, Katelaris P, Ngu M (1993) The effect ofGiardia lamblia trophozoites on trypsin, chymotrypsin and amylase in vitro. Parasitology 106: 233–238
Smith PD, Horsburgh CR, Brown WR (1981) In vitro studies on bile acid deconjugation and lipolysis inhibition byGiardia lamblia. Dig Dis Sci 26: 700–704
Tandon BN, Tandon RK, Satpathy BK, Shriniwas (1977) Mechanism of malabsorption in giardiasis: a study of bacterial flora and bile salt deconjugation in upper jejunum. Gut 18: 176–181
Tomkins AM, Drasar BS, Bradley AK, Williamson WA (1978) Bacterial colonisation of jejunal mucosa in giardiasis. Trans R Soc Trop Med Hyg 72: 33–36
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Magne, D., Chochillon, C., Favennec, L. et al. Intestinal lipid metabolism in suckling rats infected withGiardia duodenalis . Parasitol Res 80, 528–530 (1994). https://doi.org/10.1007/BF00932702
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00932702