Abstract
Immunomagnetic sorting, sequential filtrations, and counterflow centrifugal elutriation were compared for their ability to obtain enriched populations ofPneumocystis carinii developmental stages from infected rat-lung homogenates. Elutriation combined with sequential filtrations resulted in highly (>95%) enriched populations ofP. carinii cysts and trophozoites with excellent viability. This approach offers advantages over previously described methods of obtaining enrichedP. carinii cell populations and should have important applications to research on this organism.
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This study was supported by grants R01-AI-32889 (to H.v.H.), R01-AI-31702 (to P.D.W.), and R01-HL-46653 (to P.D.W.) and contract N01-AI-25139 (to P.D.W.) from the National Institutes of Health (NIH) and by the Medical Research Service, Department of Veterans Affairs, University of Cincinnati
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De Stefano, J.A., Foy, J.M., Sullivan, D.W. et al. Fractionation ofPneumocystis carinii developmental stages by counterflow centrifugal elutriation and sequential filtrations. Parasitol Res 80, 1–9 (1994). https://doi.org/10.1007/BF00932616
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DOI: https://doi.org/10.1007/BF00932616