Parasitology Research

, Volume 76, Issue 7, pp 573–577 | Cite as

Evidence for surface-coat localisation of a monoclonal antibody-isolated merozoite antigen ofBabesia divergens

  • Caroline M. Winger
  • Elizabeth U. Canning
  • Robert H. Hartley
  • Andrea Gunn
Original Investigations

Abstract

The localisation of monoclonal antibody-derived merozoite antigens ofBabesia divergens was examined using immunogold electron microscopy and immunoprecipitation of the monoclonal antibody with both biosynthetically and surface-labelled parasites. Immunogold labelling provided evidence that the antigens are components of the surface coat of the merozoite. Immunoprecipitation with biosynthetically labelled parasites showed the antigens to be of parasite origin, whereas surface labelling confirmed that the antigens form part of the surface coat.

Keywords

Electron Microscopy Monoclonal Antibody Surface Coat Immunogold Labelling Immunogold Electron Microscopy 

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Caroline M. Winger
    • 1
  • Elizabeth U. Canning
    • 1
  • Robert H. Hartley
    • 1
  • Andrea Gunn
    • 1
  1. 1.Department of BiologyImperial College of Science, Technology and MedicineLondonUK

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