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Parasitology Research

, Volume 79, Issue 4, pp 272–278 | Cite as

Processing of food vacuoles in the parasitic ciliateIchthyophthirius multifiliis after exit from the host

  • A. Lobo-da-Cunha
  • C. Azevedo
Original Investigations

Abstract

The digestive cycle of the fish parasiteIchthyophthirius multifiliis (Ciliophora) can be divided into three main stages. During stage A the vacuoles are not yet condense. This stage can be subdivided into an early phase in which food vacuoles contain almost intact fish cells and a later phase in which dense material accumulates at the periphery of the vacuoles. At stage B, food vacuoles attain a very high density, and at stage C the vacuole expands when the membrane pulls away from a condensed mass of substances in digestion. After its exit from the host the parasite encysts and divides, but new food vacuoles are not formed during this phase of the life cycle. Type A vacuoles are the first to disappear after exit from the host. The percentage of type B vacuoles increases during the first few hours of free life, decreasing later when the percentage of type C vacuoles starts to increase. At the end of the division phase, type C vacuoles are the most common. Food-vacuole egestion was observed only 20 h after exit from the host. At the theront stage, food vacuoles were not evident, but small vacuoles with acid phosphatase activity were observed.

Keywords

Life Cycle Early Phase Phosphatase Activity Acid Phosphatase Dense Material 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Allen RD (1978) Membranes of ciliates: ultrastructure, biochemistry and fusion. In: Post G, Nicolson GL (eds) Membrane fusion. North-Holland, Amsterdam, pp 657–763Google Scholar
  2. Allen RD, Fok AK (1983) Nonlysosomal vesicles (acidosomes) are involved in phagosome acidification inParamecium. J Cell Biol 97:566–570Google Scholar
  3. Allen RD, Fok AK (1984) Stages of digestive vacuoles inParamecium: membrane surface differences in location. Eur J Cell Biol 35:149–155Google Scholar
  4. De Jong ASH, Hak TJ, Van Duijin P, Daems TH (1979) A new dynamic model system for the study of capture reactions for diffusible compounds in cytochemistry: II. Effect of the composition of the incubation medium on the trapping of phosphate ions in acid phosphatase cytochemistry. Histochem J 11:145–161Google Scholar
  5. Elliott AM, Clemmons GL (1966) An ultrastructured study on ingestion and digestion inTetrahymena pyriformis. J Protozool 13:311–323Google Scholar
  6. Esteve J-C (1970) Distribution of acid phosphatase inParamecium caudatum: its relations with the process of digestion. J Protozool 17:24–35Google Scholar
  7. Ewing MS, Lynn ME, Ewing SA (1986) Critical periods in development ofIchthyophthirius multifiliis (Ciliophora) populations. J Protozool 33:388–391Google Scholar
  8. Fok AK, Allen RD (1981) AxenicParamecium caudatum: II. Changes in fine structure with culture age. Eur J Cell Biol 25:182–192Google Scholar
  9. Fok AK, Lee Y, Allen RD (1982) The correlation of digestive vacuole pH and size with the digestive cycle inParamecium caudatum. J Protozool 29:409–414Google Scholar
  10. Fok AK, Muraoka JH, Allen RD (1984) Acid phosphatase in the digestive vacuoles and lysosomes ofParamecium caudatum: a time study. J Protozool 31:216–220Google Scholar
  11. Fok AK, Ueno MS, Azada EA, Allen RD (1987) Phagosomal acidification inParamecium: effects on lysosomal fusion. Eur J Cell Biol 43:412–420Google Scholar
  12. Hoefsmit ECM, Eastermans IL, Korn C, Van Duijin P (1985) False localization of acid phosphatase activity in the nuclear envelope and endoplasmic reticulum of peritoneal macrophages. Histochem J 17:235–241Google Scholar
  13. Hopsu-Havu VK, Arstila AV, Helminen HJ, Kalimo HO (1967) Improvements in the method for electron microscopic localization of arylsulphatase activity. Histochemie 8:54–64Google Scholar
  14. Lobo-da-Cunha A, Azevedo C (1988) Ultrastructural and cytochemical study of food vacuoles and primary lysosomes inIchthyophthirius multifiliis (Ciliophora). Eur J Protistol 24:60–63Google Scholar
  15. Lobo-da-Cunha A, Azevedo C (1990) Ultrastructural localization of phosphatases with cerium in a ciliated protozoan. Acta Histochem Cytochem 23:467–473Google Scholar
  16. McArdle EW, Bergquist BL, Ehret CF (1980) Structural changes inTetrahymena rostrata during induced encystment. J Protozool 27:388–397Google Scholar
  17. McCartney JB, Fortner GW, Hansen MF (1985) Scanning electron microscopic studies of the life cycle ofIchtyhophthirius multifiliis. J Parasitol 71:218–226Google Scholar
  18. Mislan TW, Smith-Somerville HE (1986) Food vacuole morphology and membrane retrieval in the microstomal form ofTetrahymena vorax. J Protozool 33:172–179Google Scholar
  19. Nigrelli RF, Pokorny KS, Ruggieri GD (1976) Notes onIchhyophthirius multifiliis, a ciliate parasitic on freshwater fishes with some remarks on possible physiological races and species. Trans Am Microsc Soc 95:607–613Google Scholar
  20. Nilsson JR (1977) On food vacuoles inTetrahymena pyriformis GL. J Protozool 24:502–507Google Scholar
  21. Hilsson JR (1979) Phagotrophy inTetrahymena. In: Levandowsky M, Huntner SH (eds) Biochemistry and physiology of Protozoa, 2nd edn, vol 2. Academic Press, New York, pp 339–379Google Scholar
  22. Nilsson JR (1987) Structural aspects of digestion ofEscherichia coli inTetrahymena. J Protozool 34:1–6Google Scholar
  23. Smith-Somerville HE (1989) Correlation of digestive stage with changes in amphiphilic phagosomal proteins ofTetrahymena vorax. Eur J Cell Biol 49:48–54Google Scholar
  24. Weidner E, Sibley D (1985) Phagocytized intracellular microsporidian blocks phagosome acidification and phagosome-lysosome fusion. J Protozool 32:311–317Google Scholar

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • A. Lobo-da-Cunha
    • 1
  • C. Azevedo
    • 1
  1. 1.Department of Cell Biology, Institute of Biomedical SciencesUniversity of OportoPortoPortugal

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