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Archives of Gynecology and Obstetrics

, Volume 243, Issue 3, pp 157–164 | Cite as

Interaction between pregnancy zone protein and plasmin

  • O. M. Poulsen
  • J. Hau
Originals

Summary

Pregnancy zone protein (PZP, α2-PAG, SP3) was found to bind to plasmin in crossed affino-immunoelectrophoresis using sodium caseinate in the first dimension gel. The plasmin presence in the PZP-plasmin complex was confirmed by addition of antiserum against plasminogen to the gel. In crossed affino-immunoelectrophoresis using plasmin in the first dimension gel a non migrative PZP immunoreactive peak appeared, similar to the peak obtained with casein in the first dimension gel. Incubation of mixtures of PZP and plasmin also demonstrated complex formation between PZP and plasmin. The complex between PZP and plasmin could be precipitated not only by anti-PZP antibodies, but also by anti-plasminogen antibodies, confirming the interaction between the two molecules. The significance of the binding between plasmin and PZP remains to be elucidated, but it is tempting to speculate that PZP, present on the trophoblastic surface, immobilizes plasmin, rendering this molecule able to perform a local fibrinolytic activity.

Key words

Pregnancy zone protein Pregnancy associated α2-glycoprotein Enzyme binding Plasmin Complex formation 

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References

  1. Birkeland SA, Teisner B, Schilling W, Kemp E, Thomsen Pedersen G, Svehag S-E (1979) Effect of pregnancy zone protein on leucocyte migration inhibition, lymphocyte transformation and rosette formation by lymphocytes. Acta Pathol Microbiol Scand [C] 235–240Google Scholar
  2. Bohn H, Winckler W (1976) Isolierung und Charakterisierung des schwangerschafts-assoziierten α2-Glykoproteins (α2-PAG). Blut 33:377–388PubMedGoogle Scholar
  3. Chemnitz J, Hau J, Svendsen P, Folkersen J, Westergaard JG, Christensen BC (1982) Immunohistochemical demonstration of human and murine pregnancy-associated serum proteins in maternal and placental tissue. Bibl Anat 22:87–92PubMedGoogle Scholar
  4. Damber M-G (1976) On the occurrence and biological role of PZ-the “pregnancy zone” protein. Umeå University Medical Dissertations, New Series No. 13Google Scholar
  5. Damber M-G, Sandström B, von Schoultz B, Stigbrand T (1979) A new and sensitive method for quantifying and comparing the biological potency of various estrogens in man. Acta Obstet Gynecol Scand 58:527–530PubMedGoogle Scholar
  6. Fröhlander N, Gidley-Baird AA, Hau J, von Schoultz B (1987) Effects of growth hormone on pregnancy-associated murine protein-1. J Reprod Fertil 79:367–371PubMedGoogle Scholar
  7. Hau J, Westergaard JG, Svendsen P, Bach A, Teisner B (1981) Comparison of pregnancy-associated murine protein-1 and human pregnancy zone protein. J Reprod Immunol 3:341–349PubMedGoogle Scholar
  8. Hau J, Westergaard JG, Svendsen P, Teisner B, Bach A, Thomsen Pedersen G (1982) The development of a murine model for the study of human pregnancy zone protein (PZP, α2-PAG) and pregnancy-specific β1-glycoprotein (SP-1, PSβG). Placenta [Suppl] 4:51–65PubMedGoogle Scholar
  9. Hau J, Teisner B, Svendsen P (1983) Placental affinity for pregnancy-associated murine protein-1 examined by crossed affino immunoelectrophoresis. Electrophoresis 4:369–370Google Scholar
  10. Hellgren M, Blombäck M (1981) Studies on blood coagulation and fibrinolysis in pregnancy, during delivery and in the puerperium. I. Normal condition. Gynecol Obstet Invest 12:141–154PubMedGoogle Scholar
  11. Maki M, Soga K, Seki H (1980) Fibrinolytic activity during pregnacy. Tohoku J Exp Med 132: 349–354PubMedGoogle Scholar
  12. Paternoster D, Grella P, Canova P, Maggino T, Valente S, Menighetti M (1980) Modifications of coagulation factors during normal and pathological pregnancies. Clin Exp Obstet Gynecol 7:112–115PubMedGoogle Scholar
  13. Poulsen OM, Hau J (1988) Affinity of α-fetoprotein for casein analysed by affino immunoelectrophoresis and affinity chromatography. Int J Fetomaternal Med (in press)Google Scholar
  14. Sand O, Folkersen J, Westergaard JG, Sottrup-Jensen L (1985) Characterization of human pregnancy zone protein. Comparison with human α2-macroglobulin. J Biol Chem 260:15723–15735PubMedGoogle Scholar
  15. von Schoultz B, Stigbrand T, Tärnvik A (1973) Inhibition of PHA-induced lymphocyte stimulation by the pregnancy zone protein. FEBS Letters 38:23–26PubMedGoogle Scholar
  16. von Schoultz B, Stigbrand T (1974) Characterization of the “pregnancy zone protein” in relation to other α2-globulins of pregnancy. Biochim Biophys Acta 359:303–310PubMedGoogle Scholar
  17. Smithies O (1959) Zone electrophoresis in starch gels and its application to studies of serum proteins. Adv Protein Chem 14:65–113PubMedGoogle Scholar
  18. Stimson WH (1975) Pregnancy-associated α-macroglobulin (PAM) and non-specific immunosuppression in human pregnancy. Behring Inst. Mitteilungen 57:42–49Google Scholar
  19. Svendsen P, Stigbrand T, Teisner B, Folkersen J, Damber M-G, von Schoultz B, Kemp E, Svehag S-E (1978) Immunosuppressive effect of human pregnancy zone protein on H-2 incompatible mouse heart allografts. Acta Pathol Microbiol Scand [C] 86:199–201Google Scholar
  20. Than GN, Csaba IF, Szabó DG, Paál M, Ambrus M, Bajtai G (1975) In vitro suppression effect of pregnancy-associated α2-glycoproteins on the lymphocyte blastogenic response. Int Res Comm system Med Sci 3:309Google Scholar
  21. Weeke B (1973) Crossed immunoelectrophoresis. Scand J Immunol 2 [Suppl] 1:47–56Google Scholar
  22. Weiner CP, Kwaan H, Hauck WW, Duboe FJ, Paul M, Wallemark C-B (1984) Fibrin generation in normal pregnancy. Obstet Gynecol 64:46–48PubMedGoogle Scholar
  23. Westergaard JG, Bach A, Teisner B, Hau J, Grudzinskas JG (1982) Circulating human pregnancy zone protein oestriol in twin pregnancies. J Reprod Fertil 66:695–698PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • O. M. Poulsen
    • 1
  • J. Hau
    • 2
    • 3
  1. 1.Department of Obstetrics and GynaecologyUniversity of OdenseDenmark
  2. 2.Department of Veterinary PathologyLaboratory Animal UnitFrederiksberg
  3. 3.The Royal Veterinary and Agricultural UniversityCopenhagenDenmark

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