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Immunology and morphology studies on the proliferation of in vitro cultivatedEchinococcus multilocularis metacestodes

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Abstract

The larval stage ofEchinococcus multilocularis causes alveolar echinococcosis (AE) in various mammals, including humans. Traditionally metacestodes are maintained in the laboratory by serial transplantation passages into susceptible animals such as mice or gerbils. However, in animal models it has always been difficult to draw definite conclusions about the factors modulating metacestode differentiation, and investigations on gene expression and respective regulation have been hampered by the complexicity of the host-parasite interplay. This paper describes the maintenance and proliferation ofE. multilocularis metacestodes as well as the formation of protoscolices in a chemically defined medium devoid of host influence. The interactive role of a heterologous human cell line (CACO2) in the in vitro development of metacestodes was also assessed. The morphology and ultrastructure of in vitro-generated metacestodes was studied using scanning (SEM) and transmission electron microscopy (TEM). Different cultivation procedures were analyzed in terms of expression of B-and T-cell epitopes and of the relevant laminated layer-antigen Em2; the exact localization of this antigen was further demonstrated by immunogold electron microscopy.

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References

  • Aggarwal A, Merrit JW, Nash TE (1989) Cysteine-rich variant surface proteins ofGiardia lamblia. Mol Biochem Parasitol 32:39–48

    Google Scholar 

  • Alkarmi TO, Alshakarchi Z, Behbehani K (1988)Echinococcus multilocularis: the non-specific binding of different species of immunoglobulins to alveolar hydatid cysts grown in vivo and in vitro. Parasite Immunol 10:443–457

    Google Scholar 

  • Dao L (1985) An improved method of antigen detection on nitro-cellulose: in situ staining of alkaline phosphatase conjugated antibody. J Immunol Methods 82:225–231

    Google Scholar 

  • Delabre I, Gabrion C, Contant F, Petavy AF, Deblock S (1987) The susceptibility of the mongolian gerbil (Meriones unguiculatus) and the OF1 mouse strain toEchinococcus multilocularis — ultrastructural aspects of the cysts. Int J Parasitol 17:773–780

    Google Scholar 

  • Deplazes P, Gottstein B (1991) A monoclonal antibody againstEchinococcus multilocularis Em2 antigen. Parasitology 103:41–49

    Google Scholar 

  • Eckert J, Thompson RCA, Mehlhorn H (1983) Proliferation and metastases formation of larvalEchinococcus multilocularis. I. Animal model and histological findings. Z Parasitenkd 69:737–748

    Google Scholar 

  • Gottstein B (1985) Purification and characterization of a specific antigen fromEchinococcus multilocularis. Parasite Immunol 7:201–212

    Google Scholar 

  • Gottstein B, Tsang VCW, Schantz PM (1986) Demonstration of species-specific and cross-reactive components ofTaenia solium metacestode antigens. Am J Trop Med Hyg 35:308–313

    Google Scholar 

  • Gottstein B, Deplazes P, Aubert M (1992)Echinococcus multilocularis: immunological study on the “Em2-positive” laminated layer during in vitro and in vivo post-oncospheral and larval development. Parasitol Res 78:291–297

    Google Scholar 

  • Gottstein B, Eckert J, Frey H (1983) Serological differentiation betweenEchinococcus granulosus andE. multilocularis infections in man. Z Parasitenkd 69:347–356

    Google Scholar 

  • Gottstein B, Wunderlin E, Tanner I (1994)Echinococcus multilocularis: Parasite-specific humoral and cellular immune response subsets in mouse strains susceptible (AKR, C57BL/6J) or “resistant” (C57BL/10) to secondary alveolar echinococcosis. Clin Exp Immunol 96:245–252

    Google Scholar 

  • Hemphill A, Affolter M, Seebeck T (1992) A novel microtubule binding motif identified in a high molecular weight microtubule-associated protein fromTrypanosoma brucei. J Cell Biol 117:95–103

    Google Scholar 

  • Kennedy JR, Williams RW, Gray JP (1989) Use of Peldri II (a fluorocarbon solid at room temperature) as an alternative to critical point drying for biological tissues. J Electron Microsc Tech 11:117–125

    Google Scholar 

  • Mehlhorn H, Eckert J, Thompson RCA (1983) Proliferation and metastases formation of larvalEchinococcus multilocularis. II. Ultrastructural investigations. Z Parasitenkd 69:749–763

    Google Scholar 

  • Nahhas SA, Gabrion C, Walbaum S, Petavy F (1991) In vivo cultivation ofEchinococcus multilocularis protoscolices in micropore chambers. Int J Parasitol 21:383–386

    Google Scholar 

  • Rausch R, Jentoft VL (1957) Studies on the helminth fauna of Alaska. XXXI. Observations on the propagation of the larvalEchinococcus multilocularis. Leuckart, 1863, in vitro. J Parasitol 43:1–8

    Google Scholar 

  • Sakamoto T, Sugimura M (1970) Studies on echinococcosis. XXIII. Electron microscopical observations on histogenesis of larvalEchinococcus multilocularis. Jpn J Vet Res 18:131–144

    Google Scholar 

  • Smith M, Croft S (1991) Embedding and thin-section preparation: In: Harris JR (ed) Electron microscopy in biology. A practical approach. IRL-Press, Oxford, pp 17–37

    Google Scholar 

  • Thompson RCA, Lymbery AJ (1988) The nature, extent and significance of variation within the genusEchinococcus. Adv Parasitol 27:209–258

    Google Scholar 

  • Yamashita J, Ohbayashi M, Sakamoto T, Orihara M (1962) Studies on echinococcosis. XIII. Observations on the vesicular development of the scolex ofE. multilocularis in vitro. Jpn J Vet Res 10:85–96

    Google Scholar 

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Hemphill, A., Gottstein, B. Immunology and morphology studies on the proliferation of in vitro cultivatedEchinococcus multilocularis metacestodes. Parasitol Res 81, 605–614 (1995). https://doi.org/10.1007/BF00932028

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  • DOI: https://doi.org/10.1007/BF00932028

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