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Evidence of two genetic entities inBothriocephalus funiculus (Cestoda) detected by arbitrary-primer polymerase chain reaction random amplified polymorphic DNA fingerprinting

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Abstract

The genetic diversity of two samples of Cestoda (Bothriocephalus funiculus, Renaud and Gabrion, 1984) parasitizing two sympatric teleostean species was assessed using random amplified polymorphic DNA (RAPD). A total of 72Bothriocephalus were analyzed individually, and electrophoretic analysis of the amplification products of 65 primers among the 68 tested revealed monomorphic patterns, reflecting the close genetic relatedness within and between the parasites of the two samples. However, 3 primers showed polymorphic patterns at 6 RAPD sites. Analysis of the distribution of these genomic fragments, assuming random mating, showed strong linkage disequilibria (only 8 genetic combinations were observed among the 32 expected). Two genetic entities displaying a high degree of host specificity were evidence within our two samples ofB. funiculus. This powerful molecular technique can be used as a diagnostic tool in studies concerning the biodiversity of related genetic entities and could have broad applications in parasitology.

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Authors and Affiliations

  1. Laboratoire de Parasitologie Comparée (URA 698, CNRS), Université Montpellier II, Pl. E. Bataillon, 34095, Montpellier Cedex 5, France

    Olivier Verneau, Frédéric Thomas & François Renaud

  2. Centre de Recherches de Biochimie Macromoléculaire (UPR 9008, CNRS, Route de Mende, BP 5051, 34033, Montpellier Cedex, France

    Anne de Meeüs

  3. Institut des Sciences de l'Evolution (URA 327, CNRS), Université Montpellier Il, Pl. E. Bataillon, 34095, Montpellier Cedex 5, France

    François Catzeflis

Authors
  1. Olivier Verneau
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  2. Frédéric Thomas
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  3. Anne de Meeüs
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  4. François Catzeflis
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  5. François Renaud
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Verneau, O., Thomas, F., de Meeüs, A. et al. Evidence of two genetic entities inBothriocephalus funiculus (Cestoda) detected by arbitrary-primer polymerase chain reaction random amplified polymorphic DNA fingerprinting. Parasitol Res 81, 591–594 (1995). https://doi.org/10.1007/BF00932026

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  • DOI: https://doi.org/10.1007/BF00932026

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