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Physico-chemical conditions necessary for the in vitro excystment ofzygocotyle lunata (Trematoda: Paramphistomatidae)

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Abstract

Various physico-chemical factors associated with the in vitro excystment of metacercariae ofZygocotyle lunata were studied. Metacercariae could excyst with or without the presence of trypsin or bile salts, although the rate of excystation was slower in the absence of both. Crude ox-bile extract promoted faster excystment than did more refined sodium taurocholate. Pretreatment of cysts with pepsin also resulted in faster excystment rates. Optimal excystation occurred at 40°C and between pH 5.5 and 7.5. The broad pH and temperature ranges and the non-specific enzyme/bile salt requirements for excystation were correlated with the wide range of vertebrate hosts used by this trematode.

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Authors and Affiliations

  1. Department of Biological and Biomedical Sciences, University of Ulster, Shore Rd., BT37 OQB, Newtownabbey, Co. Antrim, Northern Ireland

    S. W. B. Irwin & Marian B. O'Kane

  2. Department of Biology, Lafayette College, 18042, Easton, Pennsylvania, USA

    B. Fried

Authors
  1. S. W. B. Irwin
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  2. Marian B. O'Kane
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  3. B. Fried
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Irwin, S.W.B., O'Kane, M.B. & Fried, B. Physico-chemical conditions necessary for the in vitro excystment ofzygocotyle lunata (Trematoda: Paramphistomatidae). Parasitol Res 79, 416–420 (1993). https://doi.org/10.1007/BF00931832

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  • DOI: https://doi.org/10.1007/BF00931832

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