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Ultrastructural hepatic alterations in hamsters and jirds after experimental infection with the liver flukeOpisthorchis viverrini

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Abstract

Changes in the hepatocytes of male hamsters (Mesocricetus auratus) and jirds (Meriones unguiculatus) at 220 days after experimental infection with the liver flukeOpisthorchis viverrini were studied by light and electron microscopy. The hepatocytes of the control group were characterized by an intracellular compartmentation. A globular nucleus was located centrally. The main features of the perinuclear zone were the cisternae of the rough endoplasmic reticulum (RER) and interjacent mitochondria, lysosomes, and peroxisomes. The peripheral cell region was dominated by glycogen fields and scattered lipid droplets, which were surrounded by anastomosing tubules of the smooth endoplasmic reticulum (SER). An immense proliferation of the SER was striking in the hepatocytes of animals infected withO. viverrini. Coincidentally, the intracellular compartmentation disappeared. Glycogen rosettes, RER, lysosomes, and lipid droplets were distributed irregularly all over the cell, the latter being observed more frequently than in control animals. The nuclei showed lobe-like protrusions and were enlarged. The mitochondria were often dumbbell-shaped and showed pathologic degenerations up to lysis. Our results resemble those of numerous investigations concerning hepatocellular alterations caused byN-nitroso compounds. Therefore, these observations suggest a synergistic effect for trematode infection andN-nitroso compounds in the pathogenesis of opisthorchiasis. The cellular alterations observed in the hepatocytes ofOpisthorchis-infected animals together with the accumulation of intermediate filaments seen in the adjacent bile-duct epithelia and in the epithelium of the gallbladder seem to indicate a disturbance of the cell metabolism and might be related to a neoplastic transformation.

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Abbreviations

bd :

bile duct

be :

bile-duct epithelium

c :

bile canaliculus

fl :

fluke

gd :

Golgi apparatus

gl :

glycogen

gr :

granulocyte

H :

hepatocyte

if :

intermediate filaments

li :

lipid droplet

lys :

lysosome

m :

mitochondrion

mv :

microvilli

mvb :

multivesicular body

n :

nucleus

nu :

nucleolus

pc :

periductual connective tissue

RER :

rough endoplasmic reticulum

SER :

smooth endoplasmic reticulum

sg :

secretory granules

si :

sinusoid

v :

vacuole

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Author notes

  1. R. Adam

    Present address: Fachgebiet Parasitologie (Institut für Zoologie), Universität Hohenheim, Emil-Wolff-Strasse 34, W-700, Stuttgart 70, Germany

Authors and Affiliations

  1. Zoologisches Institut der Universität Heidelberg, W-6900, Heidelberg, Germany

    R. Adam & V. Storch

  2. Abteilung Parasitologie, Hygiene-Institut, Universität Heidelberg, W-6900, Heidelberg, Germany

    E. Hinz

  3. Department of Parasitology, Faculty of Medicine, Khon Kaen University, 40002, Khon Kaen, Thailand

    P. Sithithaworn & V. Pipitgool

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  1. R. Adam
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  4. V. Pipitgool
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  5. V. Storch
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Adam, R., Hinz, E., Sithithaworn, P. et al. Ultrastructural hepatic alterations in hamsters and jirds after experimental infection with the liver flukeOpisthorchis viverrini . Parasitol Res 79, 357–364 (1993). https://doi.org/10.1007/BF00931823

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  • DOI: https://doi.org/10.1007/BF00931823

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