Parasitology Research

, Volume 79, Issue 5, pp 353–356 | Cite as

Entamoeba histolytica: changes in the zymodeme of cloned nonpathogenic trophozoites cultured under different conditions

  • Miguel Angel Vargas
  • Esther Orozco
Original Investigations


In this paper we report the occurrence of changes in the migration of certain isoenzymes of a cloned strain (MAV-CINVESTAV) ofEntamoeba histolytica. This strain was isolated from an asymptomatic carrier and showed an initial nonpathogenic zymodeme I. The transfer of polyxenic trophozoites from Robinson's medium (7% serum) to Jones' medium (30% serum) provoked changes in isoenzyme expression, resulting in the conversion of zymodeme I to a zymodeme that was similar to the XVII zymodeme except for two slow-running bands and a single fast-running band that were detected for hexokinase (HK). This XVII-like zymodeme reverted to zymodeme I when trophozoites were cultured under monoxenic conditions in TY1-S-33 medium (10% serum). When we cultured cloned strain MAV-CINVESTAV under axenic conditions in TY1-S-33 medium, trophozoites expressed a pathogenic zymodeme with two fast-running HK bands and a betaphosphoglucomutase band. In addition, phagocytosis and the ability of the trophozoites to destroy cell-culture monolayers were expressed only in trophozoites cultured under axenic conditions. The axenization procedure required the presence of liveFusobacterium symbiosum and is also described herein.


Hexokinase Asymptomatic Carrier Entamoeba Histolytica Axenic Condition Isoenzyme Expression 
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l-malate: NADP+ oxidoreductase


glucose phosphate isomerase














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  1. Andrews BJ, Mentzoni L, Bjorvatn B (1990) Zymodeme conversion of isolates ofEntamoeba histolytica. Trans R Soc Trop Med Hyg 84:63–65PubMedGoogle Scholar
  2. Brumpt E (1925) Etude sommarie de l'Entamoeba distar n. sp. amibe a kystes quadrinuclees, parasite de l'homme. Bull Acad Natl Med (Paris) 94:942–952Google Scholar
  3. Clark CG, Diamond LS (1991) Ribosomal RNA genes of “pathogenic” and “nonpathogenic”Entamoeba histolytica are distinct. Mol Biochem Parasitol 49:297–302PubMedGoogle Scholar
  4. Diamond LS, Harlow DR, Cunnick CC (1978) A new medium for the axenic cultivation ofEntamoeba histolytica and otherEntamoeba. Trans R Soc Trop Med Hyg 72:431–432PubMedGoogle Scholar
  5. Edman U, Meraz MA, Rausser S, Agabian N, Meza I (1990) Characterization of an immuno-dominant variable surface antigen from pathogenic and nonpathogenicEntamoeba histolytica. J Exp Med 172:879–888PubMedGoogle Scholar
  6. Mann BJ, Torian BE, Vedvick TS, Petri WA Jr (1991) Sequence of a cystein-rich galactose-specific lectin ofEntamoeba histolytica. Proc Natl Acad Sci USA 88:3248–3252PubMedGoogle Scholar
  7. Mirelman D (1987) Ameba-bacterium relationship in amebiasis. Microbiol Rev 51:272–284PubMedGoogle Scholar
  8. Mirelman D, Bracha R, Wexler A, Chayen A (1986a) Changes in isoenzyme patterns of a cloned culture of nonpathogenicEntamoeba histolytica during axenization. Infect Immun 54:827–832PubMedGoogle Scholar
  9. Mirelman D, Bracha R, Chayen A, Aust-Kettis A, Diamond LS (1986b)Entamoeba histolytica: effect of growth conditions and bacterial associated on isoenzyme patterns and virulence. Exp Parasitol 62:142–148PubMedGoogle Scholar
  10. Orozco E, Guarneros G, Martinez-Palomo A, Sanchez T (1983)Entamoeba histolytica: Phagocytosis as a virulence factor. J Exp Med 158:1511–1521PubMedGoogle Scholar
  11. Orozco E, Suarez ME, Sanchez T (1985) Differences in adhesion, phagocytosis and virulence of clones fromEntamoeba histolytica, strain HM1: IMSS. Int J Parasitol 15:655–660PubMedGoogle Scholar
  12. Robinson GL (1968) The laboratory diagnosis of human parasitic ameba. Trans R Soc Trop Med Hyg 62:285–293PubMedGoogle Scholar
  13. Rodriguez MA, Hernandez F, Santos L, Valdez A, Orozco E (1989)Entamoeba histolytica: Molecules involved in the target cell-parasite relationship. Mol Biochem Parasitol 37:87–100PubMedGoogle Scholar
  14. Sargeaunt PG, Williams JE, Grene JD (1978) The differentiation of invasive and noninvasiveEntamoeba histolytica by isoenzyme electrophoresis. Trans R Soc Trop Med Hyg 72:519–521PubMedGoogle Scholar
  15. Sargeaunt PG, Baveja UK, Nanda R, Anand BS (1984) Influence of geographical factors in the distribution of pathogenic zymodeme ofEntamoeba histolytica: identification of zymodeme XIV in India. Trans R Soc Trop Med Hyg 78:96–101PubMedGoogle Scholar
  16. Tannich E, Horstmann RD, Knobloch J, Arnold HH (1989) Genomic DNA differences between pathogenic and nonpathogenicEntamoeba histolytica. Proc Natl Acad Sci USA 86:5118–5122PubMedGoogle Scholar
  17. Tannich E, Ebert F, Horstmann RD (1991) Primary structure of the 170 kDa surface lectin of pathogenicEntamoeba histolytica. Proc Natl Acad Sci USA 88:1849–1853PubMedGoogle Scholar
  18. Vargas MA, Isibasi A, Kumate J, Orozco E (1990) Non-pathogenicEntamoeba histolytica: functional and biochemical characterization of a monoxenic strain. Mol Biochem Parasitol 40:193–202PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Miguel Angel Vargas
    • 1
  • Esther Orozco
    • 1
  1. 1.Department of Experimental PathologyCINVESTAV I.P.N.Mexico

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