Abstract
In this paper we report the occurrence of changes in the migration of certain isoenzymes of a cloned strain (MAV-CINVESTAV) ofEntamoeba histolytica. This strain was isolated from an asymptomatic carrier and showed an initial nonpathogenic zymodeme I. The transfer of polyxenic trophozoites from Robinson's medium (7% serum) to Jones' medium (30% serum) provoked changes in isoenzyme expression, resulting in the conversion of zymodeme I to a zymodeme that was similar to the XVII zymodeme except for two slow-running bands and a single fast-running band that were detected for hexokinase (HK). This XVII-like zymodeme reverted to zymodeme I when trophozoites were cultured under monoxenic conditions in TY1-S-33 medium (10% serum). When we cultured cloned strain MAV-CINVESTAV under axenic conditions in TY1-S-33 medium, trophozoites expressed a pathogenic zymodeme with two fast-running HK bands and a betaphosphoglucomutase band. In addition, phagocytosis and the ability of the trophozoites to destroy cell-culture monolayers were expressed only in trophozoites cultured under axenic conditions. The axenization procedure required the presence of liveFusobacterium symbiosum and is also described herein.
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Abbreviations
- HK:
-
hexokinase
- ME:
-
l-malate: NADP+ oxidoreductase
- GPI:
-
glucose phosphate isomerase
- PGM:
-
phosphoglucomutase
- P:
-
pathogenic
- NP:
-
nonpathogenic
- px:
-
polyxenic
- mx:
-
monoxenic
- ax:
-
axenic
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Vargas, M.A., Orozco, E. Entamoeba histolytica: changes in the zymodeme of cloned nonpathogenic trophozoites cultured under different conditions. Parasitol Res 79, 353–356 (1993). https://doi.org/10.1007/BF00931822
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DOI: https://doi.org/10.1007/BF00931822