Parasitology Research

, Volume 78, Issue 3, pp 209–214 | Cite as

Ultrastructural aspects ofTrypanosoma (Megatrypanum) freitasi in co-cultivation with mammalian cells

  • Neide Thomaz
  • Maurílio J. Soares
  • Wanderley de Souza
  • Maria P. Deane
Original Investigations

Abstract

Cultures ofTrypanosoma (Megatrypanum) freitasi with L929 mouse fibroblasts at 27.5°C were examined by scanning and transmission electron microscopy in an attempt to clarify the processes of colony formation by the epimastigotes and of their attachment to substrata. It was seen that the flagellates occupy intercellular spaces and do not associate with intact fibroblasts. As the trypanosome population increases, ever larger portions of the substratum are cleared of fibroblasts and occupied by conglomerates of epimastigote colonies consisting of about a dozen organisms that attach to the substratum by their anterior extremities and form pyramidal clusters. Attachment of the epimastigotes involves the flagellar membrane, which becomes extraordinarily enlarged and assumes various aspects of broad sheets, filaments and loops over the substratum or along the flagellum, which exhibits a shortened axoneme. Desmosome or hemidesmosome plaques are present when the attachment takes place between membranes or between the membrane and the substratum.

Keywords

Electron Microscopy Transmission Electron Microscopy Mammalian Cell Colony Formation Intercellular Space 

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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Neide Thomaz
    • 1
  • Maurílio J. Soares
    • 2
  • Wanderley de Souza
    • 3
  • Maria P. Deane
    • 1
  1. 1.Department of ProtozoologyInstituto Oswaldo CruzRio de JaneiroBrazil
  2. 2.Department of Ultrastructure and Cellular BiologyInstituto Oswaldo CruzRio de JaneiroBrazil
  3. 3.Instituto de BiofisicaUniversidade Federal do Rio de JaneiroRio de JaneiroBrazil

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