Parasitology Research

, Volume 78, Issue 3, pp 209–214 | Cite as

Ultrastructural aspects ofTrypanosoma (Megatrypanum) freitasi in co-cultivation with mammalian cells

  • Neide Thomaz
  • Maurílio J. Soares
  • Wanderley de Souza
  • Maria P. Deane
Original Investigations


Cultures ofTrypanosoma (Megatrypanum) freitasi with L929 mouse fibroblasts at 27.5°C were examined by scanning and transmission electron microscopy in an attempt to clarify the processes of colony formation by the epimastigotes and of their attachment to substrata. It was seen that the flagellates occupy intercellular spaces and do not associate with intact fibroblasts. As the trypanosome population increases, ever larger portions of the substratum are cleared of fibroblasts and occupied by conglomerates of epimastigote colonies consisting of about a dozen organisms that attach to the substratum by their anterior extremities and form pyramidal clusters. Attachment of the epimastigotes involves the flagellar membrane, which becomes extraordinarily enlarged and assumes various aspects of broad sheets, filaments and loops over the substratum or along the flagellum, which exhibits a shortened axoneme. Desmosome or hemidesmosome plaques are present when the attachment takes place between membranes or between the membrane and the substratum.


Electron Microscopy Transmission Electron Microscopy Mammalian Cell Colony Formation Intercellular Space 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Baltz T, Baltz D, Giroud C, Crockett J (1985) Cultivation in a semi-defined medium of animal infective forms ofTrypanosoma brucei T. equiperdum, T. evansi, T. rhodesiense andT. gambiense. EMBO J 4:1273–1277Google Scholar
  2. Bonaldo MC, Souto-Padron T, De Souza W, Goldenberg S (1988) Cell-substrate adhesion duringTrypanosoma cruzi differentiation. J Cell Biol 106:1349–1358Google Scholar
  3. Brooker BE (1971) Flagellar adhesion ofCrithidia fasciculata to Millipore filters. Protoplasma 72:19–25Google Scholar
  4. Carvalho TU, De Souza W (1987) Effect of phorbol-12 myristate 13-acetate (PMA) on the fine structure ofTrypanosoma cruzi and its interaction with activated and resident macrophages. Parasitol Res 74:11–17Google Scholar
  5. Current WL (1980)Cryptobia sp. in the snailTriadopsis multilineata (Say): fine structure of attached flagellates and their mode of attachment to the spermatheca. J Protozool 27:278–287Google Scholar
  6. Deane MP, Jansen AM (1986) AnotherTrypanosomo distinct fromT. cruzi multiplies in the anal glands of the opossumDidelphis marsupialis. Mem Inst Oswaldo Cruz 81:131–132Google Scholar
  7. Deane MP, Jansen AM (1990) Developmental stages ofTrypanosoma (Megatrypanum) freitasi Rego, Magalhães & Siqueira, 1957 in the opossumDidelphis marsupialis (Marsupialia: Didelphidae). J Protozool 37:44–47Google Scholar
  8. Gray MA, Cunningham I, Gardiner PR, Taylor AM, Luckins AG (1981) Cultivation of infective forms ofTrypanosoma congolense from trypanosomes in the proboscis ofGlossina morsitans. Parasitology 82:81–95Google Scholar
  9. Hendry KAK, Vickerman K (1988) The requirement for epimastigote tachment during division and metacyclogenesis inTrypanosoma congolense. Parasitol Res 74:403–408Google Scholar
  10. Hide G, Gray A, Harrison CM, Tait A (1989) Identification of an epidermal growth factor receptor homologue in trypanosomes. Mol Biochem Parasitol 36:51–60Google Scholar
  11. Hommel M, Robertson E (1976) In vitro attachment of trypanosomes to plastic. Experientia 32:464–466Google Scholar
  12. Maraghi S, Mchamed HA, Wallbanks KR, Molyneux DH (1987) Scratched plastic as a substrate for trypanosomatid attachment. Ann Trop Med Parasitol 81:457–458Google Scholar
  13. Mohamed HA, Maraghi S, Wallbanks KR, Molyneux DH (1988) In vitro cultivation ofHerpetosoma trypanosomes on embryonic fibroblasts and in semidefined cell-free medium. J Parasitol 74:421–426Google Scholar
  14. Molyneux DH (1969) The fine structure of the epimastigote forms ofTrypanosoma lewisi in the rectum of the flea,Nosopsyllus fasciatus. Parasitology 59:55–66Google Scholar
  15. Molyneux DH (1975)Trypanosoma (Megatrypanum) melophagium: modes of attachment of parasites to midgut, hindgut and rectum of the sheep ked,Melophagus ovinus. Acta Trop (Basel) 32:65–74Google Scholar
  16. Molyneux DH (1983) Host-parasite relationship of Trypanosomatidae in vectors. In: Harris KF (ed) Current topics in vector research, vol 1. Praeger Scientific, New York, pp 117–148Google Scholar
  17. Molyneux DH, Wallbanks KR, Ingram GA (1987) Trypanosomatid-vector interfaces—in vitro studies on parasite substrate interactions. In: Chang KP, Snary D (eds) Host-parasite cellular and molecular interactions in protozoal infections. (NATO ASI series, vol H 11) Springer, Berlin Heidelberg New York, pp 387–396Google Scholar
  18. Mungomba LM, Molyneux DH, Wallbanks KR (1989) Host-parasite relationship ofTrypanosoma corvi inOrnithomyia avicularia. Parasitol Res 75:167–174Google Scholar
  19. Rego SFM, Magalhães AEA, Siqueira AF (1957) Um novo tripanosomo do gambāTrypanosoma freitasi n. sp. Rev Bras Malariol Doenças Trop 9:277–284Google Scholar
  20. Tetley L, Vickerman K (1985) Differentiation inTrypanosoma brucei: host-parasite cell junctions and their persistence during acquisition of the variable antigen coat. J Cell Sci 74:1–19Google Scholar
  21. Thomaz N, Deane MP (1990) Continuous growth and differentiation ofTrypanosoma (Megatrypanum) freitasi Rego, Magalhães & Siqueira, 1957, in vitro. J Protozool 37:40–43Google Scholar
  22. Tieszen KL, Molyneux DH (1989) Morphology and host-parasite relationships ofCrithidia flexonema in the hindgut and malpighian tubules ofGerris odontogaster. J Parasitol 75:441–448Google Scholar
  23. Tieszen KL, Molyneux DH, Abdel-Hafez SK (1989) Host-parasite relationships and cysts ofLeptomonas lygaei inLygaeus pandurus. Parasitology 98:395–400Google Scholar
  24. Vickerman K, Preston TM (1976) Comparative cell biology of the kinetoplastid flagellates. In: Lumsden WHR, Evans DA (eds) Biology of the Kinetoplastida. Academic Press, New York, pp 35–130Google Scholar
  25. Wallbanks KR, Molyneux DH, Dirie MF (1989) Chitin derivatives as novel substrates forTrypanosoma brucei attachment in vitro. Acta Trop (Basel) 46:63–68Google Scholar
  26. Walters LL, Chaplin GL, Modi GB, Tesh RB (1989) Ultrastructural biology ofLeishmania (Viannia) panamersis inLutzomyia gomezi: a natural host-parasite association. Am J Trop Med Hyg 40:19–39Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Neide Thomaz
    • 1
  • Maurílio J. Soares
    • 2
  • Wanderley de Souza
    • 3
  • Maria P. Deane
    • 1
  1. 1.Department of ProtozoologyInstituto Oswaldo CruzRio de JaneiroBrazil
  2. 2.Department of Ultrastructure and Cellular BiologyInstituto Oswaldo CruzRio de JaneiroBrazil
  3. 3.Instituto de BiofisicaUniversidade Federal do Rio de JaneiroRio de JaneiroBrazil

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