Morphological, histochemical, and biochemical studies on the gut ofHaemonchus contortus Rud., 1803
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A study has been made on the structure and chemical composition of the gut ofHaemonchus contortus (Rud., 1803). The oesophagus has typically a triradiate, cuticle-lined lumen. The intestinal epithelium is provided with a well-developed brush border which contains periodic acid-Schiffpositive mucoproteins. The intestinal epithelium stores glycogen and lipids. It stains diffusely for phospholipids and general proteins and also for terminal-NH2 group. The presence of Fe2+ and Fe3+ containing pigments and activities of acid and alkaline phosphatases, glucose-6-phosphatase, and 5′-nucleotidase have been observed in the intestinal epithelium. Biochemically pH optimum for intestinal acid phosphatase has been found to be 4.8. The brush border shows positive reactions for acid phosphatase and glucose-6-phosphatase, and negative reactions for alkaline phosphatase and 5′-nucleotidase. The presence of enzymes in the brush border is related to extracellular digestion and absorption of nutrients.
KeywordsLipid Alkaline Phosphatase General Protein Acid Phosphatase Positive Reaction
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