Zeitschrift für Parasitenkunde

, Volume 56, Issue 3, pp 219–225 | Cite as

Characteristic protein electrophoretic patterns of fourEntamoeba strains

  • Salvador Said-Fernández
  • Rubén López-Revilla
Article

Summary

Total proteins from trophozoites of four axenized strains of the histolytica group ofEntamoeba (twoE. histolytica, oneE. invadens, and oneE. moshkovskii) were analyzed by disc-electrophoresis in polyacrylamide sodium dodecyl sulphate slab gels and by radial immunodiffusion. Each strain had a characteristic and reproducible electrophoretic pattern with about 30 major protein bands distributed between molecular weights 12,000 and 110,000. A striking similarity was found between the protein patterns of bothE. histolytica strains. Immunodiffusion also resulted in characteristic patterns, in which the greatest resemblance occurred in the same strains. The sensitivity and high resolution of the electrophoretic method for protein analysis and its correlation with antigenic characterization suggests its use as a taxonomic criterion of the histolytica group of the genusEntamoeba.

Keywords

Dodecyl Protein Band Axenized Strain Protein Analysis Characteristic Pattern 

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References

  1. Diamond, L.S.: Techniques of axenic cultivation ofEntamoeba histolytica Schaudinn, 1903 andE. histolytica-like amebae, J. Parasitol.54, 1047–1056 (1968)PubMedGoogle Scholar
  2. Gelderman, A.H., Bartgis, I.L., Keister, D.B., Diamond, L.S.: A comparison of genome sizes and thermal-denaturation-derived base composition of DNAs from several members ofEntamoeba (histolytica-group). J. Parasitol.57, 912–916 (1971)PubMedGoogle Scholar
  3. Glick, D., Good, R.A., Greenberg, L.J.: Measurement of precipitin reactions in the millimicrogram protein-nitrogen range. Science128, 1625–1626 (1958)PubMedGoogle Scholar
  4. Lewis, M.S., Piez, K.A.: Sedimentation equilibrium studies of the molecular weight of single and double chains from rat skin collagen. Biochemistry-USA3, 1126–1131 (1964)Google Scholar
  5. López-Revilla, R., Walter, G.: Polypeptide specific for cells with Adenovirus 2-SV40 hybrid Ad2+ ND1. Nature New Biol.244, 165–167 (1973)PubMedGoogle Scholar
  6. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent. J. Biol. Chem.193, 265–275 (1951)PubMedGoogle Scholar
  7. Maizel, Jr., J.V.: Polyacrylamide gel electrophoresis of viral proteins. In: Methods in virology, K. Maramorosch and H. Koprowski eds., Vol. V, p. 179–246. New York: Academic Press 1971Google Scholar
  8. Ouchterlony, Ö: Immunodifussion and immunoelectrophoresis. In: Handbook of experimental immunology, D.M. Weir ed., p. 655–706. Oxford: Blackwell Scientific Publications 1967Google Scholar
  9. Krupp, I.M.: Immunoelectrophoretic analysis of several strains ofEntamoeba histolytica. J. Trop. Med. Hyg.15, 849–854 (1966)Google Scholar
  10. Reeves, R.E., Bischoff, J.M.: Classification ofEntamoeba species by means of electrophoretic properties of amebal enzymes. J. Parasitol.54, 594–600 (1968)PubMedGoogle Scholar
  11. Reynolds, J.A., Tanford, C.: The gross conformation of protein-sodium dodecyl sulphate complexes. J. Biol. Chem.245, 5161–5165 (1970)PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • Salvador Said-Fernández
    • 1
  • Rubén López-Revilla
    • 1
  1. 1.Departamento de Biologia CelularCentro de Investigación del IPNMéxico 14México

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