Zeitschrift für Parasitenkunde

, Volume 56, Issue 3, pp 219–225 | Cite as

Characteristic protein electrophoretic patterns of fourEntamoeba strains

  • Salvador Said-Fernández
  • Rubén López-Revilla


Total proteins from trophozoites of four axenized strains of the histolytica group ofEntamoeba (twoE. histolytica, oneE. invadens, and oneE. moshkovskii) were analyzed by disc-electrophoresis in polyacrylamide sodium dodecyl sulphate slab gels and by radial immunodiffusion. Each strain had a characteristic and reproducible electrophoretic pattern with about 30 major protein bands distributed between molecular weights 12,000 and 110,000. A striking similarity was found between the protein patterns of bothE. histolytica strains. Immunodiffusion also resulted in characteristic patterns, in which the greatest resemblance occurred in the same strains. The sensitivity and high resolution of the electrophoretic method for protein analysis and its correlation with antigenic characterization suggests its use as a taxonomic criterion of the histolytica group of the genusEntamoeba.


Dodecyl Protein Band Axenized Strain Protein Analysis Characteristic Pattern 
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Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • Salvador Said-Fernández
    • 1
  • Rubén López-Revilla
    • 1
  1. 1.Departamento de Biologia CelularCentro de Investigación del IPNMéxico 14México

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