Parasitology Research

, Volume 78, Issue 5, pp 437–444 | Cite as

High osmotic pressure enables fine ultrastructural and cytochemical studies onPneumocystic carinii

I. Epon embedding
  • F. Palluault
  • C. Slomianny
  • B. Soulez
  • E. Dei-Cas
  • D. Camus
Original Investigations


High osmotic pressure was used to preserve the ultrastructure of rabbit-, SCID mouse-, and rat-derivedPneumocystis carinii organisms from osmotic stress during fixation. Organelles and cytosol were well preserved within the tonicity range of 850–1,300 mosmol. Under these experimental conditions, we determined that the endoplasmic reticulum was well developed in all parasite stages and could observe the Golgi complex, autophagic vacuoles, dense bodies, type II endoplasmic saccules, and the recently described outer surface membrane, which was found in all parasite stages. The biological implications of these findings are discussed.


Endoplasmic Reticulum Cytosol Outer Surface Osmotic Stress Surface Membrane 
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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • F. Palluault
    • 1
  • C. Slomianny
    • 1
  • B. Soulez
    • 1
    • 2
  • E. Dei-Cas
    • 1
    • 3
  • D. Camus
    • 1
    • 3
  1. 1.Unité de Biologie et Biochimie Parasitaires et Fongiques, domaine du C.E.R.T.I.A.I.N.S.E.R.M. (U. 42)Villeneuve d'AscqFrance
  2. 2.Laboratoire d'Histologie-Biologie de la reproductionFaculté de MédecineLilleFrance
  3. 3.Laboratoire de Parasitologie-MycologieFaculté de MédecineLilleFrance

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