Abstract
An AustralianBabesia bigemina vaccine strain was maintained in suspension culture for 40 days. Parasite growth was compared using two tissue-culture flask sizes (25 and 75 cm2), four gas mixes (2%, 2.5%, 3% and 3.5% O2; 5% CO2; and the balance N2) and four packed blood cell (PCV) volumes (7%, 9%, 13% and 18%). The best continuous parasite yields were obtained from suspension cultures in 75-cm2 flasks at a PCV of 13% and gas mixtures of 2%–3% O2, 5% CO2 and the balance N2. Parasite yields per millilitre of culture medium were 3 times those obtained in microaerophilous stationary-phase cultures. The method has thus far been used for 6 months to produce the Australian requirements for hveB. bigemina vaccine.
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Dalgliesh RJ, Jorgensen WK, Vos AJ de (1990) Australian frozen vaccines for the control of babesiosis and anaplasmosis in cattle —a review. Trop Anim Health Prod 22:44–52
Erp EE, Smith RD, Ristic M, Osorno BM (1980) Continuous cultivation ofBabesia bovis. Am J Vet Res 41:1141–1142
Figueroa JV, Buening GM, Kinden DA (1990) Purification of the erythrocytic stages ofBabesia bigemina cultures. Parasitol Res 76:675–680
Jorgensen WK, Vos AJ de, Dalgliesh RJ (1989) Comparison of immunogenicity and virulence betweenBabesia bigemina parasites from continuous culture and from a splenectomised calf. Aust Vet J 66:371–372
Vevy MG, Ristic M (1980)Babesia bovis: Continuous cultivation in a microaerophilous stationary phase culture. Science 207:1218–1220
Montenegro-James S, Kakoma I, Ristic M (1989) Culture derivedBabesia exoantigens as immunogens. In: Wright IG (ed) Veterinary protozoan and haemoparasite vaccines. CRC Press, Boca Raton, Florida, pp 61–67
Parker R (1973) A direct counting technique for estimating high parasitaemias in infections ofBabesia argentina, Babesia bigemina andPlasmoduim berghei. Ann Trop Med Parasitol 67:387–390
Rogers RJ, Dimmock CK, Vos AJ de, Rodwell BJ (1988) Bovine leucosis virus contamination of a vaccine prepared in vitro against bovine babesiosis and anaplasmosis. Aust Vet J 65:285–287
Timms P, Stewart NP (1989) Growth ofBabesia bovis parasites in stationary and suspension cultures and their use in experimental vaccination of cattle. Res Vet Sci 47:309–314
Vega CA, Buening GM, Green TJ, Carson CA (1985a) In vitro cultivation ofBabesia bigemina. Am J Vet Res 46:416–420
Vega CA, Buening GM, Rodriguez SD, Carson CA, McLaughlin K (1985b) Cryopreservation ofBabesia bigemina for in vitro cultivation. Am J Vet Res 46:421–423
Vega CA, Buening GM, Rodriguez SD, Carson CA (1986) Concentration and enzyme content of in vitro culturedBabesia bigemina infected erythrocytes. J Protozool 33:514–518
Vos AJ, de, Jorgensen WK (1992) Protection of cattle against babesiosis in tropical and subtropical countries with a live, frozen vaccine In: Fivaz BH, Petney TN, Horak IG (eds) Tick vector biology Medical and veterinary aspects. Springer, Berlin Heidelberg New York (in press)
Vos AJ de, Combrink MP, Bessenger R (1982)Babesia bigemina vaccine: comparison of the efficacy and safety of the Australian and South African strains under experimental conditions in South Africa. Onderstepoort J Vet Res 49:155–158
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Jorgensen, W.K., Waldron, S.J., Grath, J.M. et al. Growth ofBabesia bigemina parasites in suspension cultures for vaccine production. Parasitol Res 78, 423–426 (1992). https://doi.org/10.1007/BF00931699
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DOI: https://doi.org/10.1007/BF00931699