Abstract
The presence of carbohydrate residues in the plasma membrane of normal andTrypanosoma cruzi-infected heart muscle cells was investigated cytochemically using ruthenium red, lanthanum nitrate, periodic acid-Schiff/thiocarbahydrazide/silver, and gold-and ferritin-lectin complexes. The study combined conventional electron microscopy with the new analytical technique of electron spectroscopic imaging (ESI). Galactosyl, mannosyl, and sialyl residues were detected in regions of host-cell plasma membrane that undergo interiorization together with the parasite. Lectin-binding sites were sometimes found to show a punctate or patchy distribution in the endocytic vacuole membrane. These findings suggest the that glycoconjugates cytochemically detected in the host-cell plasma membrane participate in the invasion of heart muscle cells byT. cruzi.
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This work was supported by grants from the CNPg (Conselho Nacional de Desenvolvimento Cientifico e Tecnológico), FINEP, FAPERJ, and FIOCRUZ
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Barbosa, H.S., de Nazareth, M. & de Meirelles, S.L. Ultrastructural detection in vitro of WGA-, RCAI-, and Con A-binding sites involved in the invasion of heart muscle cells byTrypanosoma cruzi . Parasitol Res 78, 404–409 (1992). https://doi.org/10.1007/BF00931696
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DOI: https://doi.org/10.1007/BF00931696