A comparison of sporozoite and cyst merozoite surface proteins ofSarcocystis
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Surface labeling ofSarcocystis muris andS. suicanis sporozoites withN-hydroxysuccinimide biotin led to the detection of major membrane proteins with relative molecular weights of 29 and 30 kDa, respectively. Immunoblots ofSarcocystis sporozoite proteins probed with sera from infected hosts or with polyclonal monospecific antibodies generated against membrane antigens of cyst merozoites (noncorresponding stages) showed cross-reactivity between the two developmental stages (cyst merozoites and sporozoites) as well as between the speciesS. muris andS. suicanis. Two-dimensional gel electrophoresis resulted in the identification of isoforms of the sporozoite membrane antigens, with isoelectric points ranging from pH 4.7 to pH 6.4 forS. muris and from pH 4.7 to pH 5.2 forS. suicanis. The molecular masses, the charge heterogeneity, and the immunological reactivity of the surface proteins ofSarcocystis sporozoites were similar to those of cyst merozoites of both species.
KeywordsMolecular Masse Developmental Stage Membrane Protein Biotin Isoelectric Point
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