A comparison of sporozoite and cyst merozoite surface proteins ofSarcocystis
- 38 Downloads
Surface labeling ofSarcocystis muris andS. suicanis sporozoites withN-hydroxysuccinimide biotin led to the detection of major membrane proteins with relative molecular weights of 29 and 30 kDa, respectively. Immunoblots ofSarcocystis sporozoite proteins probed with sera from infected hosts or with polyclonal monospecific antibodies generated against membrane antigens of cyst merozoites (noncorresponding stages) showed cross-reactivity between the two developmental stages (cyst merozoites and sporozoites) as well as between the speciesS. muris andS. suicanis. Two-dimensional gel electrophoresis resulted in the identification of isoforms of the sporozoite membrane antigens, with isoelectric points ranging from pH 4.7 to pH 6.4 forS. muris and from pH 4.7 to pH 5.2 forS. suicanis. The molecular masses, the charge heterogeneity, and the immunological reactivity of the surface proteins ofSarcocystis sporozoites were similar to those of cyst merozoites of both species.
KeywordsMolecular Masse Developmental Stage Membrane Protein Biotin Isoelectric Point
Unable to display preview. Download preview PDF.
- Heydorn AO, Haralambidis S, Matuschka FR (1981) Zur Chemoprophylaxe und Therapie der akuten Sarkosporidiose. Berl Münch Tierärztl Wochenschr 94:229–234Google Scholar
- Horn K, Ono K, Heydorn AO (1992) In vitro excystation and cryopreservation of ovine and caprineSarcocystis species. J Protozool Res 1:13–21Google Scholar
- Lima MF, Villalta F (1989)Trypanosoma cruzi trypomastigote clones differentially express a parasite cell adhesion molecule. Mol Biochem Parsitol 33:159–170Google Scholar
- O'Donoghue PJ, Weyreter H (1984) Examinations on the serodiagnosis ofSarcocystis infections: II. Class-specific immunoglobulin responses in mice, pigs and sheep. Zentralbl Bakteriol Mikrobiol Hyg [A]:168–184Google Scholar
- Ruiz A, Frenkel JK (1976) Recognition of cyclic transmission ofSarcocystis muris by cats. J Infect Dis 133:527–531Google Scholar
- Tilley M, Upton SJ (1990) Identification of sporozoite surface proteins and antigens ofEimeria nieschulzi (Apicomplexa). J Protozool 34:86–90Google Scholar