Skip to main content
Log in

Electrical field-stimulated release of L-[G-3H]-glutamate from tissue slices of the cestodeHymenolepis diminuta

  • Short Communication
  • Published:
Parasitology Research Aims and scope Submit manuscript

Abstract

The release of preloaded [3H]-L-glutamate by tissue slices ofHymenolepis diminuta was examined by the technique of electrical field stimulation. For tissues slices in balanced saline, a high-field strength of 1.78 A resulted in a highly elevated increase in the rate of release of radiolabel, which was suppressed in calcium-free saline. This technique may prove useful for screening helminth tissues for potential neurotransmitters.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  • Kawagoe R, Onodera K, Takeuchi A (1984) The uptake and release of glutamate at the crayfish neuromuscular junction. J Physiol (Lond) 354:69–78

    Google Scholar 

  • Kihara M, Misu Y, Kubo T (1988) GABA transaminase inhibitors enhance the release of beta-alanine evoked by electrical stimulation of slices of the rat medulla oblongata. Life Sci 42:1817–1824

    Google Scholar 

  • Levi G, Patrizio M (1992) Astrocyte heterogeneity: endogenous amino acid levels and release evoked by non-N-methyl-D-aspartate receptor antagonists and by potassium-induced swelling in type-1 and type-2 astrocytes. J Neurochem 58:1943–1952

    Google Scholar 

  • Potashner SJ (1978) The spontaneous and electrically evoked release, from slices of guinea-pig cerebral cortex, of endogenous amino acids labelled via metabolism of D-[U-14C]glucose. J Neurochem 31:177–186

    Google Scholar 

  • Szerb JC, O'Regan PA (1985) Effect of glutamine on glutamate release from hippocampal slices induced by high potassium or electrical stimulation: interaction with different calcium concentrations. J Neurochem 44:1724–1731

    Google Scholar 

  • Webb RA (1986) The uptake and metabolism of L-glutamate by tissue slices of the cestodeHymenolepis diminuta. Comp Biochem Physiol [C] 85:151–162

    Google Scholar 

  • Webb RA (1988) Release of exogenously supplied [3H]glutamate and endogenous glutamate from tissue slices of the cestodeHymenolepis diminuta. Can J Physiol Pharmacol 66:889–894

    Google Scholar 

  • Webb RA, Eklove H (1989) Demonstration of intense glutamatelike immunoreactivity in the longitudinal nerve cords of the cestodeHymenolepis diminuta. Parasitol Res 75:545–548

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Webb, R.A. Electrical field-stimulated release of L-[G-3H]-glutamate from tissue slices of the cestodeHymenolepis diminuta . Parasitol Res 81, 173–174 (1995). https://doi.org/10.1007/BF00931627

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00931627

Keywords

Navigation