Abstract
The release of preloaded [3H]-L-glutamate by tissue slices ofHymenolepis diminuta was examined by the technique of electrical field stimulation. For tissues slices in balanced saline, a high-field strength of 1.78 A resulted in a highly elevated increase in the rate of release of radiolabel, which was suppressed in calcium-free saline. This technique may prove useful for screening helminth tissues for potential neurotransmitters.
References
Kawagoe R, Onodera K, Takeuchi A (1984) The uptake and release of glutamate at the crayfish neuromuscular junction. J Physiol (Lond) 354:69–78
Kihara M, Misu Y, Kubo T (1988) GABA transaminase inhibitors enhance the release of beta-alanine evoked by electrical stimulation of slices of the rat medulla oblongata. Life Sci 42:1817–1824
Levi G, Patrizio M (1992) Astrocyte heterogeneity: endogenous amino acid levels and release evoked by non-N-methyl-D-aspartate receptor antagonists and by potassium-induced swelling in type-1 and type-2 astrocytes. J Neurochem 58:1943–1952
Potashner SJ (1978) The spontaneous and electrically evoked release, from slices of guinea-pig cerebral cortex, of endogenous amino acids labelled via metabolism of D-[U-14C]glucose. J Neurochem 31:177–186
Szerb JC, O'Regan PA (1985) Effect of glutamine on glutamate release from hippocampal slices induced by high potassium or electrical stimulation: interaction with different calcium concentrations. J Neurochem 44:1724–1731
Webb RA (1986) The uptake and metabolism of L-glutamate by tissue slices of the cestodeHymenolepis diminuta. Comp Biochem Physiol [C] 85:151–162
Webb RA (1988) Release of exogenously supplied [3H]glutamate and endogenous glutamate from tissue slices of the cestodeHymenolepis diminuta. Can J Physiol Pharmacol 66:889–894
Webb RA, Eklove H (1989) Demonstration of intense glutamatelike immunoreactivity in the longitudinal nerve cords of the cestodeHymenolepis diminuta. Parasitol Res 75:545–548
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Webb, R.A. Electrical field-stimulated release of L-[G-3H]-glutamate from tissue slices of the cestodeHymenolepis diminuta . Parasitol Res 81, 173–174 (1995). https://doi.org/10.1007/BF00931627
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DOI: https://doi.org/10.1007/BF00931627