Parasitology Research

, Volume 81, Issue 2, pp 158–162 | Cite as

Cyclic nucleotide changes induced in human leukocytes by a product of axenically grownEntamoeba histolytica that inhibits human monocyte locomotion

  • G. Rico
  • O. Díaz-Guerra
  • R. R. Kretschmer
Original Paper

Abstract

Pulse exposure of human mononuclear phagocytes to the monocyte locomotion-inhibitory factor produced byEntamoeba histolytica (i.e., the 369- to 765-Da chromatographic fraction obtained from the supernatant fluid of axenically grownE. histolytica) led to a swift increase in the intracellular concentration of adenosine 3′:5′ cyclic monophosphate (cAMP). A weaker response was observed in human polymorphonuclear leukocytes, the locomotion of which, however, is not inhibited by this amebic factor. The same chromatographic fraction obtained from the axenic medium control lacked this effect, at least upon mononuclear phagocytes. On the other hand, both the monocyte locomotion-inhibitory factor and the axenic medium control, possibly through shared culture medium components, induced comparable increases in guanosine 3′:5′ cyclic monophosphate (cGMP) in human mononuclear phagocytes and in polymorphonuclear leukocytes, thus suggesting that the latter nucleotide is not critical for the leukotactic inhibitory phenomenon. Our results suggest that like other leukotactic inhibitors, the monocyte locomotion-inhibitory factor produced byE. histolytica operates through modulations of intracellular cAMP.

Keywords

Guanosine Human Monocyte Cyclic Nucleotide Polymorphonuclear Leukocyte Culture Medium Component 

Abbreviations

MP

Mononuclear phagocytes (monocytes)

MLIF

monocyte locomotion-inhibitory factor

PMN

polymorphonuclear leukocytes

cAMP

adenosine 3′:5′ cyclic monophosphate

cGMP

guanosine 3′:5′ cyclic monophosphate

PBS

phosphate-buffered (pH 7.4) normal saline

AMC

axenic medium control Gey's-A; Gey's medium with 2% albumin

ZAS

zymosan-activated serum

hpf

high-power field (400x)

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Aley SB, Scott WA, Cohn ZA (1980) Plasma membrane ofEntamoeba histolytica. J Exp Med 152:391–404Google Scholar
  2. Birnbaum L (1990) Transduction of receptor signal into modulation of effector activity by G proteins: the first 20 years or so. FASEB J 4:3178–3188Google Scholar
  3. Böyum A (1968) Isolation of mononuclear cells and granulocytes from human blood. Scand J Clin Lab Invest 21 [Suppl 97]:31–50Google Scholar
  4. Caterina MJ, Devreotes PN (1991) Molecular insights into eukaryotic chemotaxis. FASEB J 5:3078–3085Google Scholar
  5. Councilman WT, Lafleur HA (1891) Amoebic dysentery. Johns Hopkins Hosp Rep 2:395–548Google Scholar
  6. Diamond LS (1961) Axenic cultivation ofEntamoeba histolytica. Science 134:336–337Google Scholar
  7. Elsdon-Dew R (1971) Amebiasis as a world problem. Bull NY Acad Med 47:438–447Google Scholar
  8. Estensen RD, Hill HR, Quie PG, Hogan N, Goldenberg ND (1973) Cyclic GMP and cell movement. Nature 245:458–460Google Scholar
  9. Everson-Pears AG (1972) Histochemistry, theoretical and applied, vol 2. Churchill Livingstone, Edinburgh, pp 1305–1306Google Scholar
  10. Gimenez JA, Pacheco MG, Cruz E, Hernández P, Merchant MT, Kretschmer RR (1987) Ultrastructural changes associated with the inhibition of monocyte chemotaxis caused by products ofE. histolytica. Lab Invest 57:45–51Google Scholar
  11. Goldberg ND, Haddox M, Zeilig CE, Nicol SA, Acott TS, Glass DB (1976) Cyclic GMP, cyclic AMP, and the yin, yang hypothesis of biological regulation. J Invest Dermatol 67:641–645Google Scholar
  12. Harvath L, Robbins JD, Russell AA, Seamon KB (1991) cAMP and human neutrophil chemotaxis. Elevation of cAMP differentially affects chemotactic responsiveness. J Immunol 146:224–232Google Scholar
  13. Hatch GE, Nicols WK, Hill HR (1977) Cyclic nucleotide changes in human neutrophils induced by chemoattractant and chemotactic modulators. J Immunol 119:450–456Google Scholar
  14. Hill HR, Estensen RD, Quie PG, Hogan NA, Goldberg ND (1975) Modulation of human neutrophil chemotactic responses by cyclic 3′,5′-guanosine monophosphate and cyclic 3′5′-adenosine monophosphate. Metabolism 24:447–456Google Scholar
  15. Katsuki S, Arnold WP, Mittal CK, Murad F (1977) Stimulation of guanylate cyclase by sodium nitroprussiate nitroglycerin and nitric oxide in various tissue preparations and comparison to the effects of sodium azide and hydroxylamine. J Cycl Nucleot Res 3:23–25Google Scholar
  16. Kretschmer RR, López-Osuna M (1990) Effector mechanisms and immunity to amebas. In: Kretschmer RR (ed) Amebiasis: infection and disease byEntamoeba histolytica. CRC, Boca Ratón, Florida, pp 105–122Google Scholar
  17. Kretschmer RR, Collado ML, Pacheco MG, Salinas MC, López-Osuna M, Lecuona M, Castro EM, Arellano J (1985) Inhibition of human monocyte locomotion by products of axenically grownE. histolytica. Parasite Immunol 7:527–543Google Scholar
  18. Kretschmer RR, Castro EM, Rico G, Noriega R, Arellano J (1989) Further characterization of a monocyte locomotion inhibitory factor produced by axenically grownEntamoeba histolytica. Parasitol Res 75:245–246Google Scholar
  19. Kretschmer RR, Castro EM, Pacheco G, Rico G, Díaz-Guerra O, Arellano J (1991) The role of mannose in the receptor of the monocyte locomotion inhibitory factor produced byEntamoeba histolytica. Parasitol Res 77:374–378Google Scholar
  20. Marx RS, McCall CE, Bass DA (1980) Chemotaxin-induced changes in cyclic adenosine monophosphate levels in human neutrophils. Infect Immun 29:284–286Google Scholar
  21. Papierniak C, Bourey RE, Kretschmer RR, Gotoff SP, Colombetti L (1976) Technetium-99m labeling of human monocytes for chemotactic studies. J Nucl Med 17:988–992Google Scholar
  22. Pérez-Tamayo R, Brandt H (1971) Amebiasis. In: Marcial-Rojas R (ed) pathology of protozoan and helminthic diseases, Williams and Wilkins, Baltimore, pp 145–188Google Scholar
  23. Rico G, Dáz-Guerra O, Giménez-Scherer JA, Kretschmer RR (1992) Effect of the monocyte locomotion inhibitory factor (MLIF) produced byE. histolytica upon the respiratory burst of human leucocytes. Arch Med Res 23:157–159Google Scholar
  24. Rivkin I, rosenblatt J, Becker EL (1975) The role of cyclic AMP in the chemotactic responsiveness and spontaneous motility of rabbit peritoneal neutrophils. The inhibition of neutrophil movement and the elevation of cyclic AMP levels by catecholamines, postaglandins, theophylline and cholera toxin. J Immunol 115:1126–1134Google Scholar
  25. Salata RA, Pearson RD, Ravdin JI (1985) Interaction of human leucocytes andEntamoeba histolytica. Killing of virulent amebae by the activated macrophage. J Clin Invest 76:491Google Scholar
  26. Sandler JA, Clyman RI, Manganiello VC, Vaughan M (1975) The effect of serotonin (5-hydroxytryptamine) and derivatives on guanosine 3′,5′-monophosphate in human monocytes. J Clin Invest 55:431–435Google Scholar
  27. Sepúlveda B, Martínez-Palomo A (1982) Immunology of amebiasis byE. histolytica. In: Cohen S, Warren KS (eds) Immunology of parasitic disease. Blackwell, Oxford, pp 170–191Google Scholar
  28. Siegel S (1956) Nonparametric statistics for behavioral sciences. McGraw Hill, New York, pp 116–127Google Scholar
  29. Snyderman R, Altman LC, Hausman MS, Mergenhagen SE (1972) Human mononuclear leukocyte chemotaxis: a quantitative assay for humoral and cellular chemotactic factors. J Immunol 108:857–860Google Scholar
  30. Tovey RC, Oldham KG, Whelan JAM (1974) A simple direct assay for cyclic AMP in plasma and other biological samples using an improved competitive protein binding technique. Clin Chim Acta 56:221–234Google Scholar
  31. Zurier RB, Weissmann G, Hoffstein S, Kammerman S, Tai HH (1974) Mechanisms of lysosomal enzyme release from human leukocytes. II. Effects of cAMP and cGMP, autonomic agonists, and agents which affect microtubule function. J Clin Invest 53:297–309Google Scholar

Copyright information

© Springer Verlag 1995

Authors and Affiliations

  • G. Rico
    • 1
  • O. Díaz-Guerra
    • 1
  • R. R. Kretschmer
    • 1
  1. 1.Unidad de Immunología, Subjefatura de Investigación Biomédica, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social and Subdivisión de Medicina Experimental, Facultad de MedicinaUniversidad Nacional Autónoma de MéxicoMéxicoMéxico

Personalised recommendations