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Parasitology Research

, Volume 79, Issue 6, pp 497–500 | Cite as

Schizodeme and zymodeme analysis of trypanosomes of the subgenusSchizotrypanum from the bat

  • L. E. M. Teixeira
  • A. M. Gonçalves
  • A. J. Romanha
  • M. Steindel
  • A. S. Pinto
Original Investigations

Abstract

Two stocks and nine clones of trypanosomes of the subgenusSchizotrypanum were analyzed based on their electrophoretic pattern ofEcoR1 digestion products of kinetoplast DNA (k-DNA) minicricles (schizodeme) and isoenzymes (zymodeme). The trypanosome stocks were isolated fromPhyllostomus hastatus bats collected in different locations in Minas Gerais State, Brazil. The k-DNA pattern showed major variations between stocks and microvariations among the clones. Furthermore, in one of several cloning attempts, two different populations could be isolated. Among the six enzymes studied, glucose phosphate isomerase presented different patterns for stocks and clones. No similarity was found among bat trypanosomes andTrypanosoma (Schizotrypanum) cruzi standard zymodemes. This shows that bat trypanosomes are distinct fromT. (S.) cruzi.

Keywords

Glucose Phosphate Major Variation Electrophoretic Pattern Digestion Product 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • L. E. M. Teixeira
    • 1
  • A. M. Gonçalves
    • 2
  • A. J. Romanha
    • 3
  • M. Steindel
    • 4
  • A. S. Pinto
    • 1
  1. 1.Departamento de Microbiologia, Instituto de Ciências BiológicasUniversidade Federal de Minas GeraisBelo Horizonte
  2. 2.Departamento de Bioquímica e Biologia MolecularIOC-FIOCRUZRiode JaneiroBrasil
  3. 3.Centro de Pesquisas René RachouFIOCRUZBelo HorizonteBrasil
  4. 4.Departamento de Microbiologia e ParasitologiaUniversidade Federal de Santa CatarinaFlorianópolisBrasil

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