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Parasitology Research

, Volume 79, Issue 6, pp 485–487 | Cite as

Coexistence of heterogeneous populations ofToxoplasma gondii parasites within parasitophorous vacuoles of murine macrophages as revealed by a bradyzoite-specific monoclonal antibody

  • W. Bohne
  • J. Heesemann
  • U. Gross
Original Investigations

Abstract

The expression of bradyzoite-specific antigens (Bsa) ofToxoplasma gondii was studied in murine bonemarrow-derived macrophages that had previously been infected with tachyzoites. Growth conditions that allowed only restricted replication ofToxoplasma gondii resulted in heterogeneous populations; (1) Bsa-positive and Bsa-negative parasites could be observed within one parasitophorous vacuole (heterogeneous vacuole community), and (2) homogeneous Bsa-positive and homogeneous Bsa-negative vacuole communities coexisted within one macrophage host cell. These observations suggest that stage conversion does not seem to be a synchromous event for a vacuole community.

Keywords

Monoclonal Antibody Growth Condition Host Cell Heterogeneous Population Murine Macrophage 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • W. Bohne
    • 1
  • J. Heesemann
    • 1
  • U. Gross
    • 1
  1. 1.Institute of Hygiene and MicrobiologyUniversity of WürzburgWürzburgGermany

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