Abstract
An extrachromosomal nucleic acid element was detected in high-molecular-weight DNA preparations formBabesia equi merozoites. This extrachromosomal element was shown to be DNA rather than RNA and had an apparent fragment size of about 9 kilobasepairs (kb). Hybridization experiments using purified 9-kb DNA as a probe revealed sequence homologies with extrachromosomal DNA from two otherBabesia species.
This is a preview of subscription content, log in via an institution to check access.
References
Conrad PA, Iams K, Brown WC, Sohanapal B, ole-MoiYoi OK (1987) DNA probes detect genomic diversity inTheileria parva stocks. Mol Biochem Parasitol 25:213–226
Feagin JE (1992) The 6-kb element ofPlasmodium falciparum encodes mitochondrial cytochrome genes. Mol Biochem Parasitol 52:145–148
Feagin JE, Gardener MJ, Williamson DH, Wilson RJM (1991) The putative mitochondrial genome ofPlasmodium falciparum. J Protozool 38:243–245
Hall R, Coggins L, McKellar S, Shiels B, Tait A (1990) Characterisation of an extrachromosomal DNA element fromTheileria annulata. Mol Biochem Parastiol 38:253–260
Igarashi I, Aikawa M, Kreier JP (1988) Host cell-parasite interactions in babesiosis. In: M Ristic (ed) Babesiosis of domestric animals and man. CCR, Boca Raton, pp 53–69
Joseph JT, Aldritt SM, Unnasch T, Puijalon O, Wirth DF (1989) Characterization of a conserved extrachromosomal element isolated from the avian malarial parasitePlasmodium gallinaceum. Mol Cell Biol 9:3621–3629
Megson A, Inman GJ, Hunt PD, Baylis HA, Hall R (1991) The gene for apocytochrome B ofTheileria annulata resides on a small linear extrachromosomal element. Mol Biochem Parasitol 48:113–116
Mehlhorn H, Schein E (1984) The piroplasms: life cycle and sexual stages. Adv Parasitol 23:37–103
Miller SA, Dykes DD, Polesky JF (1988) A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 16:1215
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbour, New York
Schein E (1988) Equine babesiosis. In: M Ristic (ed) Babesiosis of domestic animals and man. CCR, Boca Raton, pp 197–208
Vaidya AB, Akella R, Suplik K (1989) Sequences similar to genes for two mitochondrial proteins and portions of ribosomal RNA in tandemly arrayed 6-kilobase-pair DNA of a malaria parasite. Mol Biochem Parasitol 35:97–108
Weber G (1978) Atypical mitochondria in an intracellular protozoan (Theileria annulata, Apicomplexa). Naturwissenschaften 65:601
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Schelp, C., Böse, R. & Friedhoff, K.T. Demonstration of extrachromosmal DNA fromBabesia equi merozoites. Parasitol Res 78, 707–708 (1992). https://doi.org/10.1007/BF00931526
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00931526