Parasitology Research

, Volume 81, Issue 5, pp 365–370 | Cite as

Genetic comparison ofNeospora caninum withToxoplasma andSarcocystis by random amplified polymorphic DNA-polymerase chain reaction

  • Zhi-Gang Guo
  • Alan M. Johnson
Original Paper

Abstract

To determine the relationship ofNeospora caninum to protozoa classified in the family Sarcocystidae of the phylum Apicomplexa, the genomes ofN. caninum, threeToxoplasma gondii strains (RHa, CEP, TPR) and threeSarcocystis species (S. tenella, S. muris, S. gigantea) that were thought to be closely related coccidia were compared by the random amplified polymorphic DNA (RAPD) polymerase chain reaction (PCR) technique. The genomic DNAs were amplified by the use of seven 10-mer arbitrary sequence primers to generate polymorphic DNA. Significant DNA polymorphisms were observed amongNeospora, Toxoplasma andSarcocystis. It appears that one primer tested may have value in a diagnostic RAPD-PCR to differentiateT. gondii from other closely related protozoa. The high level of genetic divergence ofN. caninum fromT. gondii strains and severalSarcocystis species observed in this study is consistent with the hypothesis thatN. caninum is indeed an independent species of protozoan parasite. As compared with theSarcocystis species tested, a closer genetic relationship ofN. caninum toT. gondii was not observed. By contrast, a closer genetic relationship ofS. muris toT. gondii was revealed in this study.

Keywords

Polymerase Chain Reaction Genetic Divergence Genomic DNAs Sequence Primer Genetic Relationship 

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Zhi-Gang Guo
    • 1
  • Alan M. Johnson
    • 1
  1. 1.Molecular Parasitology Unit, Department of Cell and Molecular BiologyUniversity of TechnologySydneyAustralia

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