Analysis of taeniid antigens using monoclonal antibodies toEchinococcus granulosus antigen 5 and antigen B
- 20 Downloads
Antigens derived fromEchinococcus granulosus, Taenia hydatigena andT. pisiformis cyst fluids,T. solium cysticerci,E. multilocularis protoscoleces andE. vogeli cyst membranes were examined by enzyme-linked immunosorbent assay (ELISA) and immunoelectrophoresis (IEP) using four monoclonal antibodies (mAb) toE. granulosus antigen 5 (Ag5) and antigen B (AgB). Anti-Ag5 mAbs 24.14 and 61A12 reacted strongly withT. hydatigena andT. pisiformis cyst fluids and, to a lesser degree, anti-AgB MAbs 31.15 and 39B3 also displayed some reaction with these antigens in ELISA. The formation of a modified arc 5 band between Anti-Ag5 mAbs andT. hydatigena cyst fluid (THCF) in IEP further confirms the existence of Ag5 inT. hydatigena cyst fluid. However, the inability of THCF andT. pisiformis cyst fluid (TPCF) to form an AgB band as well as that of TPCF to form an arc 5 band with mAbs in IEP does not exclusively prove the lack of AgB in THCF and TPCF or the lack of Ag5 in TPCF. The absence of a reaction of mAbs withT. solium, E. multilocularis andE. vogeli antigen preparations in ELISA or IEP would suggest that these mAbs may recognise epitopes different from those ofT. solium, E. multilocularis andE. vogeli parasites; this might be exploited for specific differentiation ofE. granulosus.
KeywordsMonoclonal Antibody Immunosorbent Assay Cyst Fluid Specific Differentiation Antigen Preparation
Unable to display preview. Download preview PDF.
- Capron A, Vernes A, Biguet J (1967) Le diagnostic immunoélectrophorétique de l'hydatidose. Le kyste hydatique du foie. Lyon Hydatidol, pp 27–40Google Scholar
- Varela-Díaz VM, Coltorti EA (1976) Immunodiagnostic techniques for human hydatidosis. Monograph 7. Pan American Zoonosis Centre. Pan American Health Organisation, Buenos AiresGoogle Scholar
- Yong WK, Heath DD, Parmeter SN (1978)Echinococcus granulosus, Taenia hydatigena, T. ovis: evaluation of cyst fluids as antigen for serodiagnosis of larval cestodes in sheep. NZ Vet J 26:231–234Google Scholar