Abstract
The outer part ofHymenolepis diminuta tegument was extracted with 3m KCl. The antigen was adsorbed from the extract on an affinity column containingH. diminuta-infected rat immunoglobulin immobilized on Sepharose 4B and could be eluted with 2.5m urea at pH 2.8. Analysis of polyacrylamide gel electrophoresis (PAGE) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of both the crude extract and material eluted from the column showed that the latter was markedly purified. Double diffusion and immunoelectrophoresis revealed 11 and 4 antigenic components in the crude extract and purified preparation, respectively.
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References
Befus AD (1974) The immunoglobulin coat ofHymenolepis sp. Trans R Soc Trop Med Hyg [A] 68:273
Befus AD (1977)Hymenolepis diminuta andHymenolepis microstoma: mouse immunoglobulins binding to the tegumental surface. Exp Parasitol 41:242–251
Blaska P (1966) Bestimmung der Kohlenhydrate und Lipide. Limnologica 4:403–418
Choromanski L (1980) The antigenic fractions ofHymenolepis diminuta: their usefulness in immunological examinations. Wiad Parazytol 26:571–580
Coleman RM, Carty JM, Graziadei WD (1968) Immunogenicity and phylogenic relationship of tepeworm antigens produced byHymenolepis nana andHymenolepis diminuta. Immunology 15:297–304
Davies BJ (1964) Disc electrophoresis: II. Method and application to human serum proteins. Ann NY Acad Sci 121:404–427
Grabar P, Wiliams CA (1953) Methode permetant l'étude conjugée des proprietes electrophorétiques et immunochimiques d'un melange de proteines. Application au serum sanguin. Biochim Biophys Acta 10:193–194
Harris WG, Turton JA (1973) Antibody response to tapeworm (Hymenolepis diminuta) in the rat. Nature 246:521–522
Hindsbo O, Andreassen J, Ruitenberg J (1982) Immunological and histopathological reactions of the rat against the tapewormHymenolepis diminuta and the effects of anti-thymocyte serum. Parasite Immunol 4:59–76
Hudson L, Hay F (1980) Practical immunology. Blackwell Scientific, Oxford
Isaak DD (1983) In vitro tapeworm extract-induced proliferative response of gut-associated lymphoid cells fromHymenolepis diminuta infected mice. J Helminthol 57:43–50
Knowles WJ, Oaks JA (1979) Isolation and partial biochemical characterization of the brush border plasma membrane from the cestodeHymenolepis diminuta. J Parasitol 65:715–731
Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Machnicka B (1985) Systemic suppression of the unspecific cell-mediated immunity in rats infected withHymenolepis diminuta. Wiad Parazytol 31:545–548
Machnicka B, Choromanski L (1983) Immune response and immunosuppression inHymenolepis diminuta infections in rats. Z Parasitenkd 69:239–245
Machnicka B, Krawczuk S, Walski M (1986) Influence of 0.02m EDTA and 3m KCl on surface ofHymenolepts diminuta and composition of isolated proteins. Folia Histochem Cytobiol 24:65–70
Ouchterlony O (1964) Gel diffusion techniques. In: Ackroyd JF (ed) Immunological methods. Blackwell Scientific, Oxford, pp 55–74
Roth JA, Osborne BA, Ames RS (1983) Immunoregulatory factors derived from human tumors: II. Partial purification and further immunobiochemical characterization of the human sarcoma derived immunosuppressive factor expressing HLA-DR and immunoglobulin related determinants. J Immunol 130:303–309
Weber K, Osborn M (1969) The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem 244:4406–4412
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Krawczuk, S., Rode, W. & Machnicka, B. Purification and immunologic reactivity ofHymenolepis diminuta surface antigens. Parasitol Res 76, 707–711 (1990). https://doi.org/10.1007/BF00931091
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DOI: https://doi.org/10.1007/BF00931091