Experimental infection of the leaf-monkey,Presbytis cristata, with subperiodicBrugia malayi
Presbytis cristata monkeys infected through the inoculation of between 200 and 400 subperiodicBrugia malayi infective larvae (L3) in the right thight, in both thighs or in the dorsum of the right foot were followed up for varying periods of up to about 8 months after infection. All 148 inoculated animals became patient, with mean prepatent periods being between 66 and 76 days. In animals injected in the thigh, the patterns of microfilaraemia were similar, there being a rapid rise in the geometric mean counts (GMCs) of microfilariae during the first 10–12 weeks of patency, which then plateaued at levels of >1000/ml. Adult worm recovery, expressed as the percentage of the infective dose, was significantly higher in animals injected with 100 L3 in each thigh, being 9.4% as compared with 2.8%–4.8% in other groups. It is therefore recommended that animals should be injected with 100 L3 in each thigh and that the testing of potential filaricides in this model be carried out during the phase of rapid increase in microfilaraemia to ensure that any microfilaricidal effect can easily be detected.
KeywordsAdult Worm Experimental Infection Rapid Rise Infective Larva Infective Dose
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