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Parasitology Research

, Volume 76, Issue 8, pp 662–668 | Cite as

DNA measurements and ploidy determination of different stages in the life cycle ofSarcocystis muris

  • Ute Mackenstedt
  • Dorothee Wagner
  • Alfred Otto Heydorn
  • Heinz Mehlhorn
Original Investigations

Abstract

The DNA contents of different stages within the life cycle ofSarcocystis muris were measured cytophotometrically using DNA-specific Feulgen staining. Stages of gamogony were obtained by the transfer of isolated cyst merozoites into cat kidney-cell cultures. For calculation of absolute DNA contents, the amounts of DNA in the parasites were compared with those in chicken erythrocytes, which are known. The measurements revealed that all investigated stages ofS. muris contained haploid DNA except the early zygotes, which were diploid. The further development of the zygotes started with a nuclear division, resulting in two daughter nuclei that again revealed haploid DNA values. The results confirm the existence of zygotic meiosis; thus, a haplo-homophasic life cycle is proposed for the Sarcosporidia.

Keywords

Life Cycle Nuclear Division Daughter Nucleus Chicken Erythrocyte Feulgen Staining 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Ute Mackenstedt
    • 1
  • Dorothee Wagner
    • 1
  • Alfred Otto Heydorn
    • 2
  • Heinz Mehlhorn
    • 1
  1. 1.Lehrstuhl für Spezielle Zoologie und ParasitologieRuhr-Universität BochumBochumGermany
  2. 2.Institut für Parasitologie und Tropenveterinärmedizin der Freien Universität BerlinBerlin 37

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