Skip to main content
SpringerLink
Log in

Microsporidian infection inPimpla turionellae (Ichneumonidae, Hymenoptera): characteristics and reaction with Calcofluor White

  • Original Investigations
  • Published:
Parasitology Research Aims and scope Submit manuscript

Abstract

ANosema-like microsporidian with transovarian passage was found in most organs, including muscle and nervous tissue, of newly hatched and adult wasps. The mitotic apparatus of the dikaryotic meronts includes spindle-pole bodies with intra- and extranuclear components and small kinetochores with single microtubules. Cytokinesis in stages with four nuclei involves budding. The spore wall reacts with Calcofluor White, a fluorochrome specific for β-1,4 glycosidic linkages such as those found in chitin.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Aist JR, Williams PH (1972) Ultrastructure and the time course of mitosis in the fungusFusarium oxysporum. J Cell Biol 55:368–389

    Google Scholar 

  • Becnel JJ, Hazard EL, Fukuda T (1986) Fine structure and development ofPilosporella chapmani (Microsporida: Thelohaniidae) in the mosquitoAedes triseriatus (Say). J Protozool 33:60–66

    Google Scholar 

  • Becnel JJ, Hazard EL, Fukuda T, Sprague V (1987) Life cycle ofCulicospora magna (Kudo 1920) (Microsporida: Culisporidae) inCulax restuans Theobald with special reference to sexuality. J Protozool 34:313–322

    Google Scholar 

  • Borchert A (1966) Die Krankheiten und Schädlinge der Honigbiene. Hirzel, Leipzig

    Google Scholar 

  • Brooks WM, Cranford JD (1972) Microsporidoses of the hymenopterous parasites,Campoletis sonorensis andCardiochiles nigriceps, larval parasites ofHeliothis species. J Invertebr Pathol 20:77–94

    Google Scholar 

  • Brooks WM, Hazard EL, Becnel J (1985) Two new species ofNosema (Microsporida: Nosematidae) from the Mexican bean beetleEpilachna varivestis (Coleoptera: Coccinellidae). J Protozool 32:525–535

    Google Scholar 

  • Byers B, Shriver K, Goetsch L (1978) The role of spindle pole bodies and modified microtubule ends in the initiation of microtubule assembly inSaccharomyces cerevisiae. J Cell Sci 30:331–352

    Google Scholar 

  • Günther J (1971) Entwicklungsfähigkeit, Geschlechtsverhältnisse und Fertilität vonPimpla turionellae nach Röntgenbestrahlung oder Abschnürung des Eihinterpols. Zool Jahrb Abt Anat Ontog Tiere 88:1–46

    Google Scholar 

  • Heath IB (1980) Variant mitosis in lower eukaryotes: indicators of the evolution of mitosis? Int Rev Cytol 64:1–80

    Google Scholar 

  • Kellner N, Jacobs F (1979) Präliminaruntersuchungen über Natur und Widerstandsfähigkeit der Nosemasporen (Nosema apis Zander). 27th International Beekeepers Congress, Athens. Apimondia Bucharest, p 385

  • Koehler A (1921) Über die chemische Zusammensetzung der Sporenschale vonNosema apis. Zool Anz 53:85–87

    Google Scholar 

  • Kuck A, Ruthmann A (1985) Micronuclear division and microtubular stability in the ciliateColpoda steinii. Chromosoma 93:95–103

    Google Scholar 

  • Kudo R (1920) Notes onNosema apis Zander. J Parasitol 7:85–90

    Google Scholar 

  • Lange CE (1987) A new species ofPerezia (Microsporida: Pereziidae) from the Argentine grasshopperDichroplus elongatus (Orthoptera: Acrididae). J Protozool 34:34–39

    Google Scholar 

  • Lotmar R (1943) Über den Einfluß der Temperatur auf den ParasitenNosema apis. Beih Schweiz Bienen-Z 1:261–284

    Google Scholar 

  • Middeldorf J, Ruthmann A (1984) Yeast-like endosymbionts in an ichneumonid wasp. Z Naturforsch 39c:322–326

    Google Scholar 

  • Monheit JG, Brown G, Kott MM, Schmidt WA, Moore DG (1986) Calcofluor White detection of fungi in cytopathology. Am J Clin Pathol 85:222–225

    Google Scholar 

  • Sprague V (1977) Classification and phylogeny of the microsporidia. In: Bulla LA, Cheng TE (eds) Comparative Pathology vol 2. Systematics of the Microsporidia. Plenum Press, New York

    Google Scholar 

  • Sprague V, Vernick SH (1974) Fine structure of the cyst and some sporulation stages of Ichthyosporidium (Microsporidia). J Protozool 21:667–677

    Google Scholar 

  • Vavra J (1967) Hydrolyse enzymatique des spores de mikrosporidies. J Protozool 14:205

    Google Scholar 

  • Weidner E, Byrd W, Scarborough A, Pleshinger J, Sibley D (1984) Microsporidian spore discharge and the transter of polaroblast organelle membrane into plasma membrane. J Protozool 31:195–198

    Google Scholar 

  • Yousseff NN, Hammond DM (1971) The fine structure of the developmental stages of the microsporidianNosema apis Zander. Tissue Cell 3:283–294

    Google Scholar 

  • Zander E, Böttcher FK (1984) Krankheiten der Biene, Ulmer, Stuttgart

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Lehrstuhl für Zellmorphologie, Ruhr-Universität Bochum, Postfach 1021 48, D-4630, Bochum 1, Germany

    I. Pavenstädt-Grupp & A. Ruthmann

Authors
  1. I. Pavenstädt-Grupp
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Ruthmann
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pavenstädt-Grupp, I., Ruthmann, A. Microsporidian infection inPimpla turionellae (Ichneumonidae, Hymenoptera): characteristics and reaction with Calcofluor White. Parasitol Res 76, 74–79 (1989). https://doi.org/10.1007/BF00931076

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00931076

Keywords

Search

Navigation