Abstract
A capture enzyme-linked immunosorbent assay (ELISA) for the detection ofHymenolepis diminuta coproantigen in detergent-solubilised faecal supernatants was developed. The assay was sensitive to 400 ngH. diminuta protein/ml faecal supernatant and to 30 ng/ml in PBS 0.3% Tween. Faecal antigen levels started to rise on day 7 post infection and peaked on day 20. Following drug treatment of infected rats with 10 mg/kg praziquantel, there was an initial steep rise in faecal antigen levels, followed by a decline to background levels for uninfected animals by day 7 post treatment. When assayed against faecal supernatants prepared fromH. microstoma, H. citelli, Diphyllobothrium ditrenum, Necator americanus, Strongyloides ratti andNematospiroides dubius infections as well as uninfected animals, all results from these groups were 2 SD below those for animals infected withH. diminuta. The application of such a test to important intestinal cestodiases in man is discussed.
Similar content being viewed by others
References
Baumann D, Gottstein B (1987) A double-antibody sandwich ELISA for the detection ofEntamoeba histolytica antigen in stool samples of humans. Trop Med Parasitol 38:81–85
Ellens DJ, Gielkins ALJ (1980) A simple method for the purification of 5-aminosalicylic acid. Application of the product as substrate in enzyme-linked immunosorbent assay (ELISA). J Immunol Methods 37:325–332
Ellens DJ, Leeuw PW de (1977) Enzyme-linked immunosorbent assay for the diagnosis of Rotavirus infections in calves. J Clin Microbiol 6:530–532
Flisser A (1985) Cysticercosis: a major threat to human health and livestock production. Food Technol 39:61–64
Flisser A (1988) Neurocysticercosis in Mexico. Parasitol Today 4:131–137
Green EL (1986) Immunological detection of parasite antigen in faeces. Parasitol Today 2:198–200
Green EL, Miles MA, Warhurst DC (1985) Immunodiagnostic detection ofGiardia antigen in faeces by rapid visual enzyme immunosorbent assay. Lancet II:691–693
Grundy MS (1982) Preliminary observations using a multi-layer ELISA method for the detection ofEntamoeba histolytica trophozoite antigens in stool samples. Trans R Soc Trop Med Hyg 76:396–400
Grundy MS, Voller A, Warhurst D (1987) An enzyme linked immunosorbent assay for the detection ofEntamoeba histolytica antigens in faecal material. Trans R Soc Trop Med Hyg 81:627–632
Hall A, Latham MC, Crompton DWT, Stephenson LS (1981)Taenia saginata (Cestoda) in Western Kenya: the reliability of faecal examinations in diagnosis. Parasitology 83:91–101
Hanvanich M, Viscidi R, Laughon BE, Bartlett JG, Yolken RH (1985) Stool desorbing activity: a possible cause of false positive reactions in competitive enzyme immunoassays. J Clin Microbiol 21:184–188
Jackson SG, Yip Chuck DA, Brodsky MH (1985) A double antibody sandwich enzyme immunoassay forClostridium perfringens Type A enterotoxin detection in stool specimens. J Immunol Methods 83:141–150
Kirichek VS, Nikitin AS, Frolova AA, Yarotskii LS (1986) Some aspects of the biology of the northern strain ofTaenia saginata. Med Parazitol Parazit Bolezni 6:37–39
Viscidi R, Laughon BE, Hanvanich M, Barlett JG, Yolken RH (1984) Improved enzyme immunoassays for the detection of antigens in fecal specimens. Investigation and correction of interfering factors. J Immunol Methods 67:129–143
Wilson MB, Nakane PK (1978) Recent developments in the periodate method of conjugating horseradish peroxidase (HRPO) to antibodies. In: Knapp W, Holubar K, Wicks G (eds) Immunofluorescence and related staining techniques. Elsevier, Amsterdam, pp 215–224
Yolken RH, Kim HW, Clem T, Wyatt RG, Kalica AR, Chanock RM, Kapikian AZ (1977) Enyzme linked immunosorbent assay (ELISA) for the detection of human reovirus-like agent of infantile gastroenteritis. Lancet II:263–266
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Allan, J.C., Craig, P.S. Coproantigens in gut tapeworm infections:Hymenolepis diminuta in rats. Parasitol Res 76, 68–73 (1989). https://doi.org/10.1007/BF00931075
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00931075