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Possible mode of action of toltrazuril: studies on twoEimeria species and mammalian andAscaris suum enzymes

Abstract

The anticoccidial properties of toltrazuril inEimeria falciformis-infected mice were potentiated by the simultaneous application of pyrimethamine, trimethoprim, or sulfadimidine. The same drugs potentiate the effect of toltrazuril by killingE. tenella schizonts in chicken kidney-cell cultures. Activities of some enzymes of the respiratory chain, such as succinate-cytochrome C reductase and NADH oxidase and succinate oxidase from mouse liver, were reduced in the presence of toltrazuril. The same effects could be observed when the activities of NADH oxidase and fumarate reductase from the nematodeAscaris suum were determined in the presence of the drug. Vertebrate enzymes involved in pyrimidine synthesis, e.g., dihydrofolate reductase from chicken liver, were also affected by toltrazuril; however, this effect was 500 times weaker than that shown by pyrimethamine. Toltrazuril also showed an inhibitory effect on the dihydroorotate-cytochrome C reductase from mouse liver. Our results suggest that toltrazuril primarily affects the respiratory chain and secondarily, two enzymes involved in pyrimidine synthesis.

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Dedicated to Prof. Dr. H. Debuch on the occasion of her seventieth birthday

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Harder, A., Haberkorn, A. Possible mode of action of toltrazuril: studies on twoEimeria species and mammalian andAscaris suum enzymes. Parasitol Res 76, 8–12 (1989). https://doi.org/10.1007/BF00931064

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  • DOI: https://doi.org/10.1007/BF00931064

Keywords

  • Succinate
  • Respiratory Chain
  • Fumarate
  • Trimethoprim
  • Mouse Liver