Parasitology Research

, Volume 76, Issue 6, pp 536–539 | Cite as

Induction of host resistance toRhipicephalus appendiculatus in rabbits: Effects of immunizing with detergent-solubilized tick tissue proteins

  • T. S. Dhadialla
  • B. Rutti
  • M. Brossard
Original Investigations


Resistance to the hard tick,Rhipicephalus appendiculatus, was induced in rabbits by immunizing them with tick tissue proteins extracted with a detergent, Triton X-100. There was 25% mortality in female ticks fed on immunized rabbits as compared with those fed on controls. Similarly, there was a 40% and 60% reduction in the engorged weight and the weight of egg batches, respectively, of ticks fed on immunized rabbits. Western blot analysis of detergent-solubilized tick tissue Western blot analysis of detergent-solubilized tick tissue proteins, carried out using immune sera, recognized a complex pattern of proteins. A strong reaction was observed with proteins with apparent molecular weights of 94000 and 40000 daltons.


Molecular Weight Western Blot Blot Analysis Western Blot Analysis Complex Pattern 
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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • T. S. Dhadialla
    • 1
  • B. Rutti
    • 2
  • M. Brossard
    • 2
  1. 1.The International Centre of Insect Physiology and EcologyNairobiKenya
  2. 2.The Institute of ZoologyUniversity of NeuchatelNeuchâtelSwitzerland

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