Abstract
The total and specific IgE response toAngiostrongylus cantonensis infection was evaluated according to host permissiveness. Total IgE levels measured by a double antibody radioimmunoassay (RIA) increased slowly in the permissive host (the rat), reaching a maximum between 4 and 8 weeks after infection. This maximum was earlier but significantly lower in the non-permissive host (the guinea pig). IgE antibodies specific for adult worms or L1 or L3 larvae ofA. cantonensis were measured by a radioallergosorbent test (RAST). In the case of adult worms and L1 antigens, specific IgE antibody levels showed large variations in relation to the duration of infection in rats. In contrast to total IgE levels, the specific IgE response to L3 larvae was lower in rats than in guinea pigs in both the serum and cerebrospinal fluid (CSF). These results suggest variations in the total vs specific IgE response according to host permissiveness or non-permissiveness toA. cantonensis infection. These results are discussed in the context of the possible participation of IgE antibodies in immune defence.
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References
Buck AA, Anderson RI, MacRae AA (1973) Serum immunoglobulin levels in five villages of the Republic of Chad and in onchocerciasis patients with and without microfilariae. Z Tropenmed Parasitol 24:21–31
Capron A, Dessaint JP, Capron M, Joseph M, Pestel J (1980) Role of anaphylactic antibodies in immunity to schistosomes. Am J Trop Med Hyg 29:849–857
Capron A, Dessaint JP, Haque A, Capron M (1982) Antibodydependent cell-mediated cytotoxicity against parasites. Prog Allergy 31:234–267
Capron M, Nogueira-Queiroz JA, Papin JP, Capron A (1984) Interactions between eosinophils and antibodies: in vivo protective role against rat schistosomiasis. Cell Immunol 83:60–72
Dessaint JP, Capron M, Bout D, capron A (1975a) Quantitative determination of specific IgE antibodies to schistosome antigens and serum IgE levels with schistosomiasis (S. mansoni orS. haematobium). Clin Exp Immunol 20:427–436
Dessaint JP, Bout D, Wattre P, Capron A (1975b) Quantitative determination of specific IgE antibodies toEchinococcus granulosus and IgE levels in sera from patients with hydatic diseases. Immunology 29:813–823
Dissous C, Capron A (1983)Schistosoma mansoni antigenic community between schistosomula surface and adult worm incubation products as a support for concomitant immunity. FEBS Lett 162:355–359
Dobbins Place J, Schroeder HR (1982) The fixation of anti-HBs Ag on plastic surfaces. J Immunol Meth 48:251–260
Hamilton RG, Hussain R, Ottesen EA, Adkinson NF (1981) The quantitation of parasite specific human IgG and IgE in sera: evaluation of a solid phase RIA and ELISA methodology. J Immunol Methods 44:101–114
Hogarth-Scott RS, Johansson SGO, Bennich H (1969) Antibodies toToxocara in the sera of visceralLarva migrans patients: the significance of raised levels of IgE. Clin Exp Immunol 5:619–625
Hussain R, Hamilton RG, Kumaraswami V, Adkinson NF, Ottesen EA (1981) IgE responses in human filariasis: I. Quantitation of filaria specific IgE. J Immunol 127:1623–1628
Ishida K, Yoshimura K (1986) Eosinophil responses of permissive and non-permissive hosts to the young adult worms ofAngiostrongylus cantonensis. Z Parasitenkd 72:661–671
Ito K, Sawada T, Sato S (1972) Increased serum IgE level in individuals infected withSchistosoma japoricum. Wuchereria bancrofti or hook-worm and the changes by treatment in schistosomiasis. Jpn J Exp Med 42:115–123
Jarrett E, Bazin H (1974) Elevation of total serin IgE in rats following helminth parasite infection. Nature 251:613–614
Jarrett EEE, Stewart DC (1972) Fotentiation of rat reaginic (IgE) antibody by helminth infection. Simultaneous potentiation of separate reagins. Immunology 23:749–755
Joseph M, Auriault C, Capron A, Vorng H, Viens P (1983) A new function for platelets: IgE-dependent killing of schistosomes. Nature 303:810–812
Karlsson T, Ellerson JR, Dahlbom I, Bennich H (1979) Analysis of the serum IgE levels in non-immunized rats of various strains by a radioimmunoassay. Scand J Immunol 9:217–228
Neyrinck JL, Dessaint JP, Auriault C, Capron A (1986) Measurement of picogram quantities of rat IgE from in vitro culture supernatants of B lymphocytes in experimental helminth infections. Int Arch Allergy Appl Immunol 80:342–346
O'Donnell IJ, Mitchell GP (1980) An investigation of the antigens ofAscaris lumbricoides using a radioimmunoassay and sera of naturally infected humans. Int Arch Allergy Appl Immunol 61:213–219
Orr TSC, Blair AMJN (1969) Potentiated reagin response to egg albumin and conalbumin inNippostrongylus brasiliensis infected rats. Life Sci 8:1073–1077
Ouaissi A, des Moutis I, Cornette J, Pierce RJ, Capron A (1983) Detection of IgE antibodies in onchocerciasis using a semipurified fraction fromDipetalonema viteae total antigen. Int Arch Allergy Appl Immunol 70:231–237
Pérez O, Capron M, Lastre M, Venge P, Khalife J, Capron A (1989)Angiostrongylus, cantonensis: role of eosinophils in the neurotoxic syndrome (Gordon-like phenomenon). Exp Parasitol (in press)
Phillips SM, Bentley AG, Linette G, Doughty BL, Capron M (1983) The immunologic response of congenitally athymic rats toSchistosoma mansoni infection: I. In vivo studies of resistance. J Immunol 131:1466–1474
Rousseaux-Prévost R, Bazin H, Capron A (1977) IgE in experimental schistosomiasis: I. Serum IgE levels after infection bySchistosoma mansoni in various strains of rats. Immunology 33:501–505
Rousseaux-Prévost R, Capron M, Bazin H, Capron A (1978) Quantitative determination of specific IgE antibodies againstSchistosoma mansoni: a follow-up study of two strains of infected rats. Correlation with protective immunity. Immunology 35:33–39
Rousseaux-Prévost R, Chassoux D, Bazin H, Capron A (1979) Serum IgE levels in rats infected withDipetalonema viteae L3 larvae. Clin Exp Immunol 38:389–393
Sadun EH (1972) Homocytotropic antibody response to parasitic infections. In: Soulsby EJL (ed) Immunity to animal parasites. Academic Press, New York, pp 97–129
Smithers SR, Terry RJ (1969) Immunity in schistosomiasis. Ann NY Acad Sci 162:826–840
Somorin AO, Heiner DC, Ajugwo RE (1977) Immunoglobulin E in Nigerian onchocerciasis. Am J Trop Med Hyg 26:872–876
Weiss N, Sturchler D, Dietrich FM (1978) Radioallergosorbent and indirect fluorescent antibody test in immunodiagnosis of schistosomiasis. Lancet ii:1231–1233
Weiss N, Speiser F, Hussain R (1981) IgE antibodies in human onchocerciasis. Application of a newly developed radioallergosorbent test (RAST). Acta Trop 38:353–362
Yong WK, Dobson C (1982) Antibody responses in rats infected withAngiostrongylus cantonensis and the passive transfer of protective immunity with immune serum. Z Parasitenkd 67:329–336
Yoshimura K, Soulsby EJL (1976)Angiostrongylus cantonensis: lymphoid cell responsiveness and antibody production in rats. Am J Trop Med Hyg 25:99–107
Yoshimura K, Yamagishi T (1975) Immunologic response of guinea pigs to infection withAngiostrongylus cantonensis. Jpn J Vet Sci 37:585–591
Yoshimura K, Yamagishi T (1976) Reaginic antibody productions in rabbits and rats infected withAngiostrongylus cantonensis. Jpn J Vet Sci 38:33–40
Yoshimura K, Aiba H, Hayasaki M, Yoshida H (1976) Delayed hypersensitivity responses of guinea pig and rat toAngiostrongylus cantonensis infection. Jpn J Vet Sci 38:579–593
Yoshimura K, Aiba H, Oya H, Yoshiro F (1980)Angiostrongylus cantonensis: development following pulmonary arterial transfers into permissive and non-permissive hosts. Exp Parasitol 49:339–352
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Pérez, O., Lastre, M., Capron, M. et al. Total and specific IgE in serum and cerebrospinal fluid of rats and guinea pigs infected withAngiostrongylus cantonensis . Parasitol Res 75, 476–481 (1989). https://doi.org/10.1007/BF00930976
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DOI: https://doi.org/10.1007/BF00930976