Parasitology Research

, Volume 75, Issue 6, pp 449–454 | Cite as

Isolation and partial characterization of rhoptries and micronemes fromEimeria nieschulzi zoites (Sporozoa, Coccidia)

  • J. F. Dubremetz
  • E. Ferreira
  • C. Dissous
Original Investigations


Homogenization and subcellular fractionation of sporozoites ofEimeria nieschulzi have allowed the recovery of highly enriched fractions of rhoptries and micronemes. The isolated organelles kept their in situ morphological characteristics. Their apparent densities in sucrose solutions were approximately 1.18 g/cm3 for rhoptries and 1.14 g/cm3 for micronemes. When subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), the microneme fraction showed two major polypeptides at 220 and 94 kDa. The rhoptry fraction contained at least three predominant peptides at 200, 150, and 63 kDa. Micronemes were also isolated from third-genaration merozoites of the same species; their characteristics were identical to those of the organelles isolated from sporozoites.


Sodium Peptide Sucrose Polypeptide Morphological Characteristic 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Aikawa M, Miller LH, Johnson J, Rabbege J (1978) Erythrocyte entry by malarial parasites. A moving junction between erythrocyte and parasite. J Cell Biol 77:72–87Google Scholar
  2. Bontemps M, Yvore P (1974) Technique de purification de suspension de sporozoites d'Eimeria sur colonne de fibres synthetiques. Ann Rech Vet 5:109–113Google Scholar
  3. Dubremetz JF, Dissous C (1980) Characteristic proteins of micronemes and dense granules fromSarcocystis tenella zoites (Protozoa, Coccidia). Mol Biochem Parasitol 1:279–289Google Scholar
  4. Entzeroth R (1984) Electron microscope study of host parasite interaction ofSarcocystis muris (Protozoa, Coccidia) in tissue culture and in vivo. Z Parazitenkd 70:131–134Google Scholar
  5. Entzeroth R, Dubremetz JF, Hodick D, Ferreira E (1986) Immunoelectron microscopic demonstration of exocytosis of dense granule contents into the secondary parasitophorous vacuole ofSarcocystis muris (Protozoa, Apicomplexa). Eur J Cell Biol 41:182–188Google Scholar
  6. Holder AA, Freeman RR, Uni S, Aikawa M (1985) Isolation of aPlasmodium falciparum rhoptry antigen. Mol Biochem Parasitol 14:293–303Google Scholar
  7. Jensen JB, Hammond DM (1975) Ultrastructure of the invasion ofEimeria magna sporozoites into cultured cells. J Protozool 22:411–415Google Scholar
  8. Marquardt WC (1966) The living, endogenous stages of the rat coccidiumEimeria nieschulzi. J Protozool 13:509–514Google Scholar
  9. Nichols BA, Chiappino ML, O'Connor GR (1983) Secretion from the rhoptries ofToxoplasma gondii during host cell invasion. J Ultrastruct Res 83:85–98Google Scholar
  10. Puck TT, Cieciura SJ, Fisher HW (1957) Clonal growth in vitro of human cells with fibroblast morphology. Comparison of growth and genetic characteristics of single epithelioid and fibroblast like cells from a variety of human organs. J Exp Med 106:145–158Google Scholar
  11. Roger N, Dubremetz JF, Delplace P, Fortier B, Tronchin G, Vernes A (1988) Characterization of a 225 kilodalton rhoptry protein ofPlasmodium falciparum. Mol Biochem Parasitol 27:135–142Google Scholar
  12. Sadak A, Taghy Z, Fortier B, Dubremetz JF (1988) Characterization of a family of rhoptry proteins ofToxoplasma gondii. Mol Biochem Parasitol 29:203–212Google Scholar
  13. Schofield L, Bushell GR, Cooper JA, Saul AJ, Upcroft J, Kidson C (1986) A rhoptry antigen ofPlasmodium falciparum contains conserved and variable epitopes recognized by inhibitory monoclonal antibodies. Mol Biochem Parasitol 18:183–195Google Scholar
  14. Scholtyseck E, Mehlhorn H (1970) Ultrastructural study of characteristic organelles (paired organelles, micronemes, micropores) of Sporozoa and related organisms. Z Parasitenkd 34:97–127Google Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • J. F. Dubremetz
    • 1
  • E. Ferreira
    • 2
  • C. Dissous
    • 3
  1. 1.U42 INSERMVilleneuve d'AscqFrance
  2. 2.ERA 184 CNRSUniversité de Lille 1Villeneuve d'AscqFrance
  3. 3.U186 INSERMLille CedexFrance

Personalised recommendations