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Parasitology Research

, Volume 75, Issue 6, pp 449–454 | Cite as

Isolation and partial characterization of rhoptries and micronemes fromEimeria nieschulzi zoites (Sporozoa, Coccidia)

  • J. F. Dubremetz
  • E. Ferreira
  • C. Dissous
Original Investigations

Abstract

Homogenization and subcellular fractionation of sporozoites ofEimeria nieschulzi have allowed the recovery of highly enriched fractions of rhoptries and micronemes. The isolated organelles kept their in situ morphological characteristics. Their apparent densities in sucrose solutions were approximately 1.18 g/cm3 for rhoptries and 1.14 g/cm3 for micronemes. When subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), the microneme fraction showed two major polypeptides at 220 and 94 kDa. The rhoptry fraction contained at least three predominant peptides at 200, 150, and 63 kDa. Micronemes were also isolated from third-genaration merozoites of the same species; their characteristics were identical to those of the organelles isolated from sporozoites.

Keywords

Sodium Peptide Sucrose Polypeptide Morphological Characteristic 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • J. F. Dubremetz
    • 1
  • E. Ferreira
    • 2
  • C. Dissous
    • 3
  1. 1.U42 INSERMVilleneuve d'AscqFrance
  2. 2.ERA 184 CNRSUniversité de Lille 1Villeneuve d'AscqFrance
  3. 3.U186 INSERMLille CedexFrance

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