Abstract
Fresh eggs, laid bySchistosoma japonicum adult worms in vitro or recovered from tissue after trypsin digestion, as well as non-viable lyophilized eggs were injected into mice via the cecal vein. The freshly laid eggs induced a marked, maximal reaction at 2 weeks after implantation. This reaction was indistinguishable from that seen early in naturally infected mice. Lyophilized eggs induced granulomas only one-tenth the size of those formed around freshly laid eggs. Maximal granulomatous reactivity was not seen until the 4th week after implantation and the intensity of cellular reactivity and associated histopathological change was much lower than that observed with freshly laid eggs. Reactions against live, tissue-extracted eggs were quantitatively and temporally intermediate between those observed against laid and those surrounding lyophilized eggs. The results suggests that these differences in granuloma formation are due to variable quantities of locally produced cytotoxic materials and/or antigen that stimulate immune reactions of different intensities.
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References
Amano T, Oshima T (1987) Granuloma formation around inoculatedSchistosoma japonicum eggs in the livers of ddY mouse. Jpn J Parasitol 36:128–134
Botros SS, El-Badrawi N, El-Raziky EH (1986) Subcutaneous implantation of the spleen as a new technique for experimental induction of hepaticSchistosoma mansoni egg granulomas. Trans R Soc Trop Med Hyg 80:515–516
Cheever AW, Byram JE, Lichtenberg F von (1985) Immunopathology ofSchistosoma japonicum infection in athymic mice. Parasite Immunol 7:387–398
Doughty BL, Phillips SM (1982) Delayed hypersensitivity granuloma formation aroundSchistosoma mansoni eggs in vitro. 1. Definition of the model. J Immunol 128:30–36
Edungbola LD, Schiller EL (1979) Histopathology of hepatic and pulmonary granulomata experimentally induced with eggs ofSchistosoma mansoni. J Parasitol 65:253–261
Edungbola LD, Cha YN, Bueding E, Schiller EL (1982) Granuloma formation around exogenous eggs ofSchistosoma mansoni andSchistosoma japonicum in mice. Afr J Med Med Sci 11:75–79
Hang LM, Warren KS, Boros DL (1974)Schistosoma mansoni: antigenic secretions and the etiology of egg granulomas in mice. Exp Parasitol 35:288–298
Hirata M, Hieda T, Kato H, Tsutsumi H (1987) Presence of IgG inside of eggs isolated from or trapped in the liver ofSchistosoma japonicum infected rabbits. Jpn J Parasitol 36:63–67
Joky A, Cornu M, Louis D, Grimaud JA (1978) Polyvinylchloride (PVC) particle implantation in mouse liver. A technique for experimental study of schistosome egg induced liver pathology. Experientia 34:547–549
Kitaguchi T, Amano T, Oshima T (1988) Reduction of the size ofSchistosoma japonicum egg granulomas in the liver of presensitized mice. The Japan-United States Cooperative Medical Science Program, 23rd Joint Conference on Parasitic Diseases, 17–20, July, Tokyo
Lichtenberg F von (1962) Host response to eggs ofSchistosoma mansoni: 1. Granuloma formation in the unsensitized laboratory mouse. Am J Pathol 41:711–731
Lichtenberg F von, Erickson DG, Sadun EH (1973) Comparative histopathology of schistosome granulomas in the hamster. Am J Pathol 72:149–178
Litt M (1963) Studies in experimental eosinophilia: V. Eosinophils in lymph nodes of guinea pigs following primary antigenic stimulation. Am J Pathol 42:529–547
Long GW, Lewert RM, Pelley RP (1981) Characterization of the glycoprotein antigens which mediate theSchistosoma japonicum circumoval precipitin reaction. Infect Immun 34:389–396
Matsuda H, Nosenas JS, Tanaka H, Santos AT, Trinidad-Perez D (1977) Comparative studies on reading criteria of the circumoval precipitin reaction ofSchistosoma japonicum for field survey in highly endemic areas. Jpn J Exp Med 47:369–375
Moore DE, Warren KS (1967) Hepatosplenic schistosomiasis mansoni and japonica compared in mice each infected with one pair of worms. Trans R Soc Trop Med Hyg 61:104–109
Olds GR, Mahmoud, AAF (1981) Kinetics and mechanisms of pulmonary granuloma formation aroundSchistosoma japonicum eggs injected into mice. Cell Immunol 60:251–260
Stavitsky AB (1987) Immune regulation in schistosomiasis japonica. Immunol Today 8:228–233
Warren KS (1982) The secret of the immunopathogenesis of schistosomiasis: in vivo models. Immunol Rev 61:189–213
Warren KS, Domingo EO (1970) Granuloma formation aroundSchistosoma mansoni, S. haematobium andS. japonicum eggs. Size and rate of development, cellular composition, cross-sensitivity, and rate of egg destruction. Am J Trop Med Hyg 19:292–304
Warren KS, Boros DL, Hang LM, Mahmoud AAF (1975) TheSchistosoma japonicum egg granuloma. Am J Pathol 80:279–294
Weinstock JV, Boros DL (1981) Heterogeneity of the granulomatous response in the liver, colon, ileum, and ileal Peyer's patches to schistosome eggs in murine schistosomiasis mansoni. J Immunol 127:1906–1909
Weinstock JV, Boros DL (1983) Organ differences in composition and function observed in hepatic and intestinal granulomas isolated from mice with schistosomiasis mansoni. J Immunol 130:418–422
Yokogawa M, Sano M (1966) Immunosero-diagnosis of schistosomiasis japonica: II. Isolation techniques of theSchistosoma eggs from tissues for the circumoval precipitin test. Jpn J Parasitol 15:394–398
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Hirata, M., Takushima, M., Kage, M. et al. Induction of experimental murine granuloma formation againstSchistosoma japonicum eggs produced by in vitro ova deposition, in vitro tissue extraction, or lyophilization. Parasitol Res 77, 315–319 (1991). https://doi.org/10.1007/BF00930907
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DOI: https://doi.org/10.1007/BF00930907