Production of L-serine by the methanol utilizing bacterium, Pseudomonas 3ab

  • Hartmut Keune
  • Hermann Sahm
  • Fritz Wagner
Industrial Microbiology


A bacterium capable of growth on methanol and some organic acids as sole source of carbon and energy has been isolated and designated Pseudomonas 3ab. This facultative methylotrophic organism apparently utilizes the serine pathway of formaldehyde fixation.

When methanol was used as the sole carbon source for growth, L-serine production by Pseudomonas 3ab occurred upon the addition of glycine and methanol at the end of the exponential growth phase. The maximum yield of L-serine (4.7 g/l) was obtained when 20 g/l glycine and 8 g/l methanol were added and the pH of the culture medium was changed to 8.5.

Although Pseudomonas 3ab is unable to grow on L-serine or glycine, it is very active in decomposing these amino acids. The degradation of L-serine and glycine has been shown to be pH-dependent with a minimum at pH 8.5–9.0.


Methanol Formaldehyde Glycine Carbon Source Serine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • Hartmut Keune
    • 1
  • Hermann Sahm
    • 1
  • Fritz Wagner
    • 1
  1. 1.Lehrstuhl für Biochemie and Biotechnologie der TechnischenUniversität Braunschweig and Gesellschaft für Molekularbiologische Forschung mbHBraunschweig-StöckheimGermany

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