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Parasitology Research

, Volume 76, Issue 3, pp 207–213 | Cite as

Purine salvage and metabolism inBabesia bovis

  • Christina Matias
  • Susan E. Nott
  • Aldo S. Bagnara
  • William J. O'Sullivan
  • Annette M. Gero
Original Investigations

Abstract

Studies of the incorporation of radio-labelled purine precursors into the erythrocytic forms ofBabesia bovis under tissue-culture conditions have confirmed the presence in the parasite of enzymatic activities responsible for the salvage of preformed purines. The results also revealed that the parasite was capable of a variety of nucleotide interconversions, such that exogenous hypoxanthine and adenosine were incorporated into both adenine and guanine nucleotides followed by the incorporation of these nucleotides into the adenine and guanine moieties of RNA and DNA. No evidence was found for salvage of preformed pyrimidines. Evidence was also obtained for the insertion of a parasite-specific nucleoside/nucleobase transporter into the membrane of the bovine (host) red cell. Thus, whereas normal (non-parasitised) bovine red cells are essentially incapable of transporting nucleosides across their membranes, the invasion of these cells byB. bovis introduces a transporter that can be inhibited by classic nucleoside transport inhibitors.

Keywords

Nucleotide Enzymatic Activity Adenosine Adenine Pyrimidine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Christina Matias
    • 1
  • Susan E. Nott
    • 1
  • Aldo S. Bagnara
    • 1
  • William J. O'Sullivan
    • 1
  • Annette M. Gero
    • 1
  1. 1.School of BiochemistryUniversity of New South WalesKensingtonAustralia

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