Bioconversion from DL-Homoserine to L-Threonine
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The bioconversion from DL-Homoserine to L-Threonine byEnterobacter cloacae resting-cells is directed by various parameters: concentration in cells, in homoserine, in glucose, temperature, shaking, pH, and time.
Some of them, such as temperature, shaking, and pH were determined during preliminary tests.
In order to consider the reciprocal effect of the other parameters, we applied an experimental scheme based on the simultaneous variation of all parameters of the process.
The method of factorial designs is based on the choice of an experimental field for which we make a matrix of the experiments. The measured result is the yield of L-Threonine produced in relation to L-Homoserine employed.
The computational interpretation of the results gives the levels of significance of every parameter and the direction of the variation needed to improve the yield.
This method permits us to obtain information which may be interpreted immediately, with a reduced number of experiments.
KeywordsGlucose Experimental Field Factorial Design Preliminary Test Experimental Scheme
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