Abstract
Monoclonal antibodies were used in an inhibition enzymelinked immunosorbent assay (IELISA) to detect a variety of schistosome derived antigens. Preparations were obtained from various stages ofSchistosoma mansoni and from the eggs ofS. japonicum. Using appropriate titers of monoclonal antibodies it was possible to detect less than 0.01 μg/ml of schistosome antigens. The sensitivity of IELISA was dependent upon the type and concentration of the monoclonal antibody used, as well as upon the source of the antigens. Specificity studies showed that some of the monoclonal antibodies recognized species specific antigenic determinants, while others reacted against genus specific antigens. Furthermore, certain antibodies interacted with antigens which were neither genus or species specific. Fractions ofS. mansoni andS. japonicum egg antigen extracts, which have been previously considered to be relatively pure, were compared using certain monoclonal antibodies. The results indicate that these fractions are very heterogenous with regard to the unique antigenic specificities. For example, most antigenic determinants in crude soluble egg antigen are not retained on concanavalin-A lectin affinity columns and major serologic antigens have more than one determinant with different distributions. The expression of these antigenic determinants appears to be a function of both the concentration of the specific antigen and the mode of expression of the moiety within the antigenic matrix.
Similar content being viewed by others
References
Abdel-Hafez, SK, Phillips, SM, Zodda, DM (1983). Detection and characterization of schistosome derived antigens by inhibition enzyme linked immunosorbent assay (IELISA). Exp Parasitol 55:219–232
Boros, DL, Warren, KS (1970) Delayed hypersensitivity-type granuloma formation and dermal reaction induced and elicited by a soluble factor reaction isolated fromSchistosoma mansoni eggs. J Exp Med 132:488–507
Bout, D, Santoro, F, Carlier, Y, Bina, JC, Capron, A (1977) Circulating immune complexes in Schistosomiasis. Immunology 33:17–22
Carlier Y, Bout, D, Strecker, G, Derbay, H, Capron, A (1980) Purification, immunochemical, and biologic characterization of the Schistosoma circulating M antigen. J Immunol 124:2442–2450
Carter, CE, Colley DG (1978a) An electrophoretic analysis ofSchistosoma mansoni infection. J Parasitol 64:385–395
Carter, CE, Colley DG (1978b) Partial purification and characterization ofSchistosoma mansoni soluble egg antigen using ConA-Sepharose chromatography. J Immunol 122:2204–2209
Catty, D, Hassan, K, Mumo, J, Appleby, P, Hawkins, S, Adu, J, Das, P, Lavanchy, D, Braun, D (1982) Monoclonal antibodies and diagnosis of Schistosomiasis in man. 30th Protides of Biological Fluids, Brussels
Deedler, AM, Klappe, HJM, Van den Aardweg, GJMJ, Van Meerbeke, EHEM (1976)Schistosoma mansoni: Demonstration of two circulating antigens in infected hamsters. Exp Parasitol 40:189–197
Hamburger, J, Pelley, RP, Warren, KS (1976)Schistosoma mansoni soluble egg antigens. II. Determination of the stage and species specificity of their serologic activity by radioimmunoassay. J Immunol 117:1561–1566
Harrison, DJ, Carter, CE, Colley, DC (1979) Immunoaffinity purification ofSchistosoma mansoni soluble egg antigens. J Immunol 122:2210–2217
Hayunga, EG, Murrell KD, Taylor, DW, Vannier, WE (1979) Isolation and characterization of surface antigens fromSchistosoma mansoni. II. Antigenicity of radiolabeled proteins from adult worms. J Parasitol 65:497–506
Hillyer, GV, Pelley, R (1980) The major serological antigen (MSA1) fromSchistosoma mansoni eggs in a “circumoval” precipitinogen. Am J Trop Med Hyg 29:582–585
Hillyer, GV, Pelley, RP, Diaz, A (1979) Solubilization of antigens ofFasciola hepatica which react with antibodies toSchistosoma mansoni. J Parasitol 65:55–60
Houba, V, Koech, DK, Sturrock, RF, Butterworth, AE, Kusel, JR, Mahmoud, AAF (1976) Soluble antigens and antibodies in sera from baboons infected withSchistosoma mansoni. J Immunol 117:705–707
Lowry, OH, Rosebrough, NJ, Farr, AL, Randall, RJ (1951) Protein measurement with folin phenol reagent. J Biol Chem 193:265–275
Mitchell, GF, Cruise, KM, Garcia, EG, Anders, F (1981) Hybridoma-derived antibody with immunodiagnostic potential forSchistosomiasis japonica. Proceed Nation Acad of Sci U.S.A. 78:3165–3169
Nash, TG, Lunde, MN, Cheever, A (1981) Analysis and antigenic activity of a carbohydrate fraction derived from adultSchistosoma mansoni. J Immunol 126:805–810
Nash, TE, Nasir-Ud-Din, Jeanloz, RW (1977) Further purification and characterization of a circulating antigen in schistosomiasis. J Immunol 119:1627–1633
Nash, TE, Prescott, B, Neva, FA (1974) The characteristics of a circulating antigen in schistosomiasis. J Immunol 112:1500–1507
Pelley, RP, Pelley, RJ, Hamburger, J, Peters, PA, Warren KS (1976)Schistosoma mansoni soluble egg antigens. I. Identification and purification of three major antigens, and the employment of radioimmunoassay for their further characterization. J Immunol 117:1553–1560
Phillips, SM, Colley, DG (1979) Immunologic aspects of host responses to schistosomiasis: resistance, immunopathology, and eosinophil involvement. Prog in Aller 24:49–182
Phillips, SM, Reid, WA, Doughty-B, Khoury, PB (1978) The cellular and humoral immune response toSchistosoma mansoni infections in inbred rats. III. Development of optimal protective immunity following natural infections and artificial immunizations. Cell Immunol 38:225–238
Sadun, EH, Schoenbechler, MJ, Bentz, M (1965) Multiple antibody response inSchistosoma mansoni infections. Antigenic constituents in eggs, cercariae, and adults (excretions and secretions) determined by floculation reactions, cross absorption and double diffusion studies. Am J Trop Med Hyg 14:977–995
Santoro, F, Prata, A, Castro, CN, Capron, A (1980) Circulating antigens, immune complexes and C3d levels in human schistosomiasis: relationship withSchistosoma mansoni egg output. Clin Exper Immunol 42:219–225
Santoro, F, Vandemeulebrouke, B, Capron, A (1978) The use of the radio-immuno-precipitaschistosomiasis. J Immunol Meth 47:229–237
Santoro, F, Vandemeulebrouke, B, Capron, A (1979)Schistosoma mansoni circulating antigens and immune-complexes in infected mice. Exp Parasitol 47:392–402
Verwaede, C, Czych, J, Bazin, H, Capron, M, Capron, A (1979) Procution d'anticorps monoclonaux anti-Schistosoma mansoni Etude preliminaire Paris, t. 289 Serie D, 725–727
Zodda, DM, Abdel-Hafez, SK, Phillips, SM (1983) Characterization of monoclonal antibodies againstSchistosoma mansoni. Am J Trop Med Hyg 32:69–77
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Abdel-Hafez, S.K., Phillips, S.M. & Zodda, D.M. Schistosoma mansoni: Detection and characterization of schistosome derived antigens by inhibition enzyme-linked immunosorbent assay (IELISA) utilizing monoclonal antibodies. Z. Parasitenkd. 70, 105–117 (1984). https://doi.org/10.1007/BF00929580
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00929580