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Amino acid uptake in skeletal muscle of rats bearing the Yoshida AH-130 ascites hepatoma

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Abstract

Rats bearing the Yoshida AH-130 ascites hepatoma show decreased activity of neutral amino acid transport in skeletal muscle measuredin vivo as the tissue accumulation of the analogue α-amino [1-14C]isobutyrate (AIB). The decreased accumulation of AIB observed is not merely a consequence of the hypoinsulinaemia present in these animals (as a result of tumour burden) sincein vitro experiments carried out using incubations of isolated soleus muscles also showed a decreased uptake of neutral amino acids. In these preparations the addition of insulin results in similar increases in uptake both in the pair-fed controls and the tumour-bearing animals, thus suggesting similar insulin sensitivities. The decrease in amino acid uptake in soleus muscle is associated with a decrease in the activity of system A, while systems L and ASC show no particular changes as a result of the tumour growth. The kinetic characterisation of system A in the Yoshida-bearing rats shows a decrease in Vmax together with a decrease in KM in relation with the pair-fed animals.

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Authors and Affiliations

  1. Departament de Bioquímica i Fisiologia, Facultat de Biologia, Universitat de Barcelona, Barcelona, Spain

    Cèlia García-Martínez, Francisco J. López-Soriano & Josep M. Argilés

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  1. Cèlia García-Martínez
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  2. Francisco J. López-Soriano
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  3. Josep M. Argilés
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García-Martínez, C., López-Soriano, F.J. & Argilés, J.M. Amino acid uptake in skeletal muscle of rats bearing the Yoshida AH-130 ascites hepatoma. Mol Cell Biochem 148, 17–23 (1995). https://doi.org/10.1007/BF00929498

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  • DOI: https://doi.org/10.1007/BF00929498

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