Conclusions
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1.
A dynamic method was developed for measuring the isotherms for the adsorption of water vapor by chymotrypsinogen A. The obtained isotherms were compared with the isotherms that were measured under static conditions.
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2.
The values of the apparent capacity of the monolayer from the sorption and desorption isotherms, obtained by the static and dynamic methods, coincide, while the shape of the isotherms depends on the method used to achieve hydration equilibrium.
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3.
A correlation was shown to exist between the values of the apparent capacity of the monolayer and the number of surface polar groups of the chymotrypsinogen A molecule.
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Translated from Izvestiya Akademii Nauk SSSR, Seriya Khimicheskaya, No, 9, pp. 2110–2114, September, 1973.
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Klimova, V.A., Tusupkaliev, U., Sherman, F.B. et al. Hydration of chymotrypsinogen A. Russ Chem Bull 22, 2054–2057 (1973). https://doi.org/10.1007/BF00929404
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DOI: https://doi.org/10.1007/BF00929404