Skip to main content
SpringerLink
Log in

Inhibition of monoamine oxidase-A of rat brain by pyrethroids — anin vitro kinetic study

  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Abstract

Anin vitro kinetic study on inhibition of the monoamine oxidase-A (MAO-A) of the rat brain by two pyrethroids, namely permethrin (PM) and cypermethrin (CPM), has shown that PM and CPM competitively inhibit MAO-A by altering both the Michaelis-Menten constant (Km) and the maximum velocity (Vmax). Inhibitor constant values (Ki) indicated that CPM was a more effective inhibitor of MAO-A than PM. Both PM and CPM caused maximum inhibition of MAO-A at neutral pH. CPM significantly elevated the activation energy values of MAO-A as compared to those of PM.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

MAO:

Manoamine Oxidase

PM:

Permethrin

CPM:

Cypermethrin

TTV:

Toxicity Test Value

IC50 :

Inhibitor Concentration that causes 50 percent inhibition

References

  1. Fu RC, Xian-Fang Liu, Shiuan Chen: Inhibition of monoamine oxidase by 7-chloro-4-nitrobenzofurazan. J Neurochem 55:813–818, 1990

    Google Scholar 

  2. Johnston JP: Some observations upon a new inhibitor of monoamine oxidase in brain tissue. Biochem Pharmacol 17:1285–1297, 1968

    Google Scholar 

  3. Houslay MD, Tipton KF, Youdim MBH: Multiple forms of monoamine oxidase: fact and artefact. Life Sci 19:467–478, 1976

    Google Scholar 

  4. Akhilender Naidu K: Inhibition of monoamine oxidase by the fungicide metalaxyl. Journal of Toxicology and Environmental Health 27:395–398, 1989

    Google Scholar 

  5. Leung TKC, Lai JCK, Lim L: The regional distribution of monoamine oxidase activities towards different substrates: Effects in rat brain of chronic administration of manganese chloride and of ageing. J Neurochem 36:2037–2043, 1981

    Google Scholar 

  6. Knoll J: (−) Deprenyl — the MAO inhibitor without the cheese effect. Trends in Neurosciences 2:111–113, 1979

    Google Scholar 

  7. Kadir HA, Knowles CO: Inhibition of rat brain monoamine oxidase by insecticides. Gen Pharmacol 12:239–247, 1981

    Google Scholar 

  8. Elliot M (1977a): ‘Synthetic pyrethroids’, in Synthetic Pyrethroids (Elliot M ed.), American Chemical Society, Washington, DC. pp 1–28

    Google Scholar 

  9. Wouters W, Bercken JVD: Action of pyrethroids. Gen Pharmacol 9:387–398, 1978

    Google Scholar 

  10. Ghiasuddin SM, Soderlund DM: ‘Pyrethroid insecticides: Potent, stereospecific enhancers of mouse brain sodium channel activation’. Pestic Biochem Physiol 24:200–206, 1985

    Google Scholar 

  11. Eldefrawi ME, Abbassy MA, Eldefrawi AT: Effects of environmental toxicants on nicotinic acetylcholine receptors: action of pyrethroids. In: T. Narahashi (Ed.), Cellular and Molecular Neurotoxicology, Raven, New York, 1984, pp 177–189

    Google Scholar 

  12. Yoshii M, Tsunoo A, Narahashi T: Effects of pyrethroids and veratridine on two types of Ca channels in neuroblastoma cells. Soc Neurosci Absr 11:518, 1985

    Google Scholar 

  13. Casida JE, Gammon DW, Glickman AH, Lawrence LJ: Mechanisms of selective action of pyrethroid insecticides. Annu Rev Pharmacol Toxicol 23:413–438, 1983

    Google Scholar 

  14. Clark JM, Matsumura F: Two different types of inhibitory effects of pyrethroids on nerve Ca− and Ca+−Mg− ATPase activity in the squid,Loligo Paelei, Pestic Biochem Physiol 18:180–190, 1982

    Google Scholar 

  15. Cole LM, Casida JE: Pyrethroid toxicology in the frog. Pestic Biochem Physiol 20:217–224, 1983

    Google Scholar 

  16. White INH, Verschoyle RD, Moradian MH, Barnes JM: The relationship between brain levels of cismethrin and bioresmethrin in female rats and neurotoxic effects. Pestic Biochem Physiol 6:491–500, 1976

    Google Scholar 

  17. Leibowitz MD, Schwarz JR, Holan G, Hille B: Electro-physiological comparison of insecticide and alkaloid agonists of Na channels. J Gen Physiol 90:75–93, 1987

    Google Scholar 

  18. Vijverberg PMH, Bercken JVD: Neurotoxicological effects and the mode of action of pyrethroid insecticides. CRC Critical Reviews of Toxicology 21:105–126, 1990

    Google Scholar 

  19. Israel Silman: Molecular structure of acetylcholinesterase TIBS 1: 225–227, 1976

    Google Scholar 

  20. Krajl M: A rapid microfluorimetric determination of monoamine oxidase. Biochem Pharmacol 14:1683–1685, 1965

    Google Scholar 

  21. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with the Folin-phenol reagent. J Biol Chem 193:265–275, 1951

    Google Scholar 

  22. Jagannatha Rao KS:In vitro enzymatic toxicity test model validity approach. In:in vitro toxicology-approaches to validation (A. M. Goldberg (ed), No. 5, Mary Ann Liebert Inc. Publishers, New York, 1987, pp 421–451

    Google Scholar 

  23. Lineweaver H, Burk D: The determination of enzyme dissociation constants. J Am Chem Soc 56:658–666, 1934

    Google Scholar 

  24. Dixon M, Webb EC: Enzymes, 2nd Edn (Longmans Green and Co, London), 1964, p 330

    Google Scholar 

  25. Lehninger AL: Principles of Biochemistry, CBS Publishers and Distributors (India), 1984, pp 207–244

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Nutrition and Food Safety, Central Food Technological Research Institute, 570 013, Mysore, India

    Gazula Valeswara Rao & K. S. Jagannatha Rao

Authors
  1. Gazula Valeswara Rao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. S. Jagannatha Rao
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rao, G.V., Rao, K.S.J. Inhibition of monoamine oxidase-A of rat brain by pyrethroids — anin vitro kinetic study. Mol Cell Biochem 124, 107–114 (1993). https://doi.org/10.1007/BF00929202

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00929202

Key words

Search

Navigation